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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 08

Page:

1410-1417

Research Field:

Publishing date:

Info

Title:

Expression of SCRN1 in oral squamous cell cancer-associated fibroblasts and its effect on biological characterization of HSC3 cell line

Author(s):

HAN Jinpeng¹; 2; ZHENG Kaiyue³; NAN Xiaoxu¹; 2; ZHAO Runzhe¹; 2; LIU Ying¹; 2

1.Department of Stomatology，Affiliated Hospital of North Sichuan Medical College，Sichuan Nanchong 637100,China;2.Department of Oral Medicine,North Sichuan Medical College，Sichuan Nanchong 637100,China;3.Department of Stomatology，Hebei Eye Hospital,Hebei Xingtai 054001,China.

Keywords:

SCRN1; cancer-associated fibroblasts; MMP2; oral squamous cell cancer

PACS:

R739.8

DOI:

10.3969/j.issn.1672-4992.2024.08.007

Abstract:

Objective:To investigate the expression of secernin 1 (SCRN1) in cancer-associated fibroblasts (CAFs) of oral squamous cell cancer (OSCC) and its effect on the secretion of MMP2,and to further study the effect of co-culture with CAFs on migration and invasion ability of HSC3 cells.Methods:The expression of SCRN1 in OSCC and adjacent tissues was detected by immunohistochemistry (IHC).Through primary cultivated,isolated,purified and identified normal fibroblasts (NFs) and CAFs,the SCRN1 expression was detected by immunocytochemistry (ICC),Western Blot (WB) and qRT-PCR.The proliferation,migration,and invasion capabilities of NFs,CAFs and CAFs-shSCRN1 were detected by CCK-8 and Transwell assays.The supernatant of NFs and CAFs was collected and the matrix metalloproteinase 2 (MMP2) levels and activity were detected by ELISA and gelatin zymography.Then they were co-cultured with HSC3 cells respectively to explore the effects of the above two supernatant culture conditions on the migration and invasion ability of HSC3 cell line.After silencing the SCRN1 gene of CAFs,the supernatant of CAFs,CAFs-shSCRN1 and CAFs-Vector was collected and MMP2 level and activity were detected,and then the supernatant was co-cultured with HSC3 cells respectively,to investigate the effects of the three supernatant culture conditions on the migratory and invasive abilities of HSC3 cell line.Results:SCRN1 was highly expressed in OSCC tissue.NFs and CAFs were successfully isolated,cultured and identified.CAFs expressed higher levels of SCRN1 than NFs,and their proliferation,migration,and invasion abilities were stronger than those of NFs.Silencing of SCRN1 reduced proliferation,migration and invasion ability of CAFs.Co-culture with CAFs supernatant significantly promoted the migration and invasion ability of HSC3 cells and secreted higher levels and activity of MMP2.Co-culture with CAFs-shSCRN1 resulted in a markedly diminished ability to promote migration and invasion of the HSC3 cells,as well as a reduction in MMP2 levels and activity.Conclusion:SCRN1 can modulate the secretion of MMP2 by CAFs,which,in the tumor microenvironment,promotes the migration and invasion ability of HSC3 cells.

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Memo

Memo:

四川省南充市2022年市校科技战略合作专项（编号：22SXQT0356）；川北医学院附属医院第一批临床研究课题项目（编号：2021LC010）

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