|Table of Contents|

The impacts of lncRNA PSMA3-AS1 on malignant progression of ovarian cancer by regulating miR-142-3p/HMGA2 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 08
Page:
1401-1409
Research Field:
Publishing date:

Info

Title:
The impacts of lncRNA PSMA3-AS1 on malignant progression of ovarian cancer by regulating miR-142-3p/HMGA2 axis
Author(s):
SA Rihu; ZHAO Dexiong; SUN Wenping
The Second Department of Obstetrics,Qinghai Red Cross Hospital,Qinghai Xining 810000,China.
Keywords:
lncRNA PSMA3-AS1; miR-142-3p/HMGA2; cell proliferation; cell migration; cell invasion
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2024.08.006
Abstract:
Objective:To investigate the impacts and mechanism of lncRNA PSMA3-AS1 on malignant progression of ovarian cancer by regulating the miR-142-3p/HMGA2 axis.Methods:53 patients with ovarian cancer diagnosed in our hospital between March 2019 and July 2022 were selected for the study,and the patients' ovarian cancer tissues and paracancerous tissues were collected.Human normal ovarian cells HOSEpiC and ovarian cancer cell lines SKOV3,OVCAR3,A2780,COV362 were cultured in vitro.RT-qPCR was applied to detect the expression of lncRNA PSMA3-AS1 in ovarian cancer cells to screen the optimal intervention cell line.The double luciferase reporter gene experiment was applied to verify the targeting relationship between lncRNA PSMA3-AS1,HMGA2 and miR-142-3p.SKOV3 cells were separated into si-NC group,si-PSMA3-AS1 group,si-PSMA3-AS1+anti-miR-NC group,si-PSMA3-AS1+anti-miR-142-3p group,miR-NC group,miR-142-3p mimics group,miR-142-3p mimics+pcDNA group,and miR-142-3p mimics+HMGA2 group.Cell proliferation,apoptosis,migration,and invasion were detected.Western blot was applied to detect the expression of Ki67,Cyclin D1,Bcl-2,cleaved caspase 3,and HMGA2 proteins.The mouse transplantation tumor experiment was applied to verify the effect of lncRNA PSMA3-AS1 on the growth of ovarian cancer tumors.Results:Compared with paracancerous tissue,lncRNA PSMA3-AS1 and HMGA2 expression was elevated and miR-142-3p expression was decreased in ovarian cancer tissue (P＜0.05).lncRNA PSMA3-AS1 and HMGA2 had the highest expression in SKOV3 cells,and miR-142-3p had the lowest expression level in SKOV3 cells.Pull down test and double luciferase report showed that lncRNA PSMA3-AS1,HMGA2 had a targeting relationship with miR-142-3p.After knocking down lncRNA PSMA3-AS1 or overexpressing miR-142-3p,the cell OD,cell clone number,scratch healing rate,cell invasion number,and the expression of Ki67,Cyclin D1,Bcl-2,HMGA2 were obviously reduced,while cell apoptosis rate and the expression of cleaved caspase 3 were obviously increased (P＜0.05).Inhibiting the expression of miR-142-3p or overexpressing HMGA2 was able to reverse the inhibitory effects of knocking down lncRNA PSMA3-AS1 or overexpressing miR-142-3p on the malignant behavior of ovarian cancer cells.In vivo experiments showed that knocking down the expression of lncRNA PSMA3-AS1 was able to inhibit tumor growth in mice.Conclusion:lncRNA PSMA3-AS1 is highly expressed in ovarian cancer,and knocking down lncRNA PSMA3-AS1 can regulate miR-142-3p/HMGA2 axis to inhibit the malignant development of ovarian cancer.

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Memo

Memo:
青海省卫健委医药卫生指导性课题（编号：2019-wjzdx-69）
Last Update: 1900-01-01