|Table of Contents|

Alleviative effect and mechanism of ginsenoside Rg3 on breast cancer-induced bone pain in rats

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 08
Page:
1397-1401
Research Field:
Publishing date:

Info

Title:
Alleviative effect and mechanism of ginsenoside Rg3 on breast cancer-induced bone pain in rats
Author(s):
LI Yachen; SHENG Gege; WU Jiliang; ZHU Haili
School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Hubei Xianning 437100,China.
Keywords:
ginsenoside Rg3; cancer-induced bone pain; spinal inflammation; NLRP3 inflammasome; mitochondria
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.08.005
Abstract:
Objective:To investigate the alleviative effect and mechanism of ginsenoside Rg3 on breast cancer-induced bone pain in rats.Methods:A rat model of breast cancer-induced bone pain was constructed by inoculation of MRMT-1 rat breast cancer cells into the medullary cavity of tibia in rats.Rats were randomly divided into three groups,including Sham,Model and ginsenoside Rg3 (Rg3) groups.Ginsenoside Rg3 was injected on rats by the tail vein for 3 consecutive days.The changes of pain behavior of rats were detected.Molecular docking analyzed the binding between Rg3 and protein.Immunofluorescence and Western blotting assays were performed to detect the expression of proteins related to inflammation in spinal cord tissue in each group of animals.Results：Bone metastases of breast cancer caused bone damage,induced mechanical pain,thermal hyperalgesia and spontaneous pain of rats.The spinal cord glia cells and NLRP3 inflammasome were activated.The pro-inflammatory factor IL-1β level was increased.The antioxidant factors Nrf2 and GPX4 levels were decreased.The levels of mitochondrial oxidative damage related proteins DHODH and cytochrome C were increased.Ginsenoside Rg3 treatment alleviated the mechanical pain,thermal hyperalgesia and spontaneous pain of rats,inhibited spinal inflammatory reaction,enhanced spinal Nrf2/GPX4-mediated antioxidant responses,decreased spinal mitochondrial oxidative level.In addition,ginsenoside Rg3 combined with Nrf2 assayed by auto-dock.Conclusion:Ginsenoside Rg3 treatment activates Nrf2-mediated antioxidant responses,inhibits inflammatory reaction in the spinal cord,and alleviates cancer-induced bone pain.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.81971066）；国家自然科学基金资助项目（编号：81971066）
Last Update: 1900-01-01