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Effects of PUMA on the apoptosis of esophageal cancer EC109 cell line induced by pterostilbene

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2015 06
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Effects of PUMA on the apoptosis of esophageal cancer EC109 cell line induced by pterostilbene
Feng Yingtong12Liu Honggang1Pang Sainan3Yang Yang4Yan Xiaolong1Li Xiaofei1
1.Department of Thoracic Surgery,Tangdu Hospital,The Fourth Military Medical University,Shaanxi Xi'an 710038,China;2.Department of Cardiothoracic Surgery,Chinese people's Liberation Army NO.97 Hospital,Jiangsu Xuzhou 221004,China;3.Department of Cardiothoracic Surgery,the First Affiliated Hospital,Jiamusi University,Heilongjiang Jiamusi 154002,China;4.Department of Cardiovascular Surgery,Xijing Hospital,The Fourth Military Medical University,Shaanxi Xi'an 710032,China.
pterostilbeneesophageal cancerEC109 cell lineapoptosisPUMA
Objective:To investigate the effects of pterostilbene (PTE) on the viability and apoptosis of human esophageal cancer EC109 cell line,and to define the role of p53 upregulated modulator of apoptosis (PUMA) in it.Methods:Firstly,the EC109 cells were treated with PTE (0,50,100,150μmol/L),cell counting kit-8 (CCK-8) was used to measure the cell viability,Annexin V-FITC/PI staining was used to quantify the apoptosis of EC109 cells,Caspase-3 activity was measured using a colorimetric assay kit,the measurement of intracellular ROS was based on the ROS-mediated conversion of non-fluorescent 2',7'-DCFH-DA into fluorescent DCFH,and Western bolt was used to detect the expression of PUMA.Secondly,the EC109 cells were treated with PTE (0,100μmol/L) for 24h after they were transfected with control or PUMA siRNA,then Western bolt was used to detect the expression of PUMA,CCK-8 was used to measure the cell viability.Results:Being treated with PTE (0,50,100,150μmol/L) for 12,24 and 36h,the cell viability of EC109 cells was decreased in a dose-and time-dependent manner(P<0.01).After the treatment of PTE for 24h,The apoptotic index,Caspase-3 activity and ROS generation were increased (P<0.01).Furthermore,when the expression of PUMA was decreased after siRNA transfection,the cytotoxic effects of PTE on EC109 cells decreased(P<0.01).Conclusion:PTE treatment can effectively inhibit cell viability of EC109 cells,and the mechanism is related to the apoptosis induced by PUMA,PTE may be a potential new drug for treatment of esophageal cancer.


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Last Update: 2015-02-02