|Table of Contents|

Effects of PUMA on the apoptosis of esophageal cancer EC109 cell line induced by pterostilbene

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
731-735
Research Field:
Publishing date:

Info

Title:
Effects of PUMA on the apoptosis of esophageal cancer EC109 cell line induced by pterostilbene
Author(s):
Feng Yingtong1; 2; Liu Honggang1; Pang Sainan3; Yang Yang4; Yan Xiaolong1; Li Xiaofei1
1.Department of Thoracic Surgery,Tangdu Hospital,The Fourth Military Medical University,Shaanxi Xi'an 710038,China;2.Department of Cardiothoracic Surgery,Chinese people's Liberation Army NO.97 Hospital,Jiangsu Xuzhou 221004,China;3.Department of Cardiothoracic Surgery,the First Affiliated Hospital,Jiamusi University,Heilongjiang Jiamusi 154002,China;4.Department of Cardiovascular Surgery,Xijing Hospital,The Fourth Military Medical University,Shaanxi Xi'an 710032,China.
Keywords:
pterostilbene; esophageal cancer; EC109 cell line; apoptosis; PUMA
PACS:
R73-36+1;R735.1
DOI:
10.3969/j.issn.1672-4992.2015.06.01
Abstract:
Objective:To investigate the effects of pterostilbene (PTE) on the viability and apoptosis of human esophageal cancer EC109 cell line,and to define the role of p53 upregulated modulator of apoptosis (PUMA) in it.Methods:Firstly,the EC109 cells were treated with PTE (0,50,100,150μmol/L)，cell counting kit-8 (CCK-8) was used to measure the cell viability,Annexin V-FITC/PI staining was used to quantify the apoptosis of EC109 cells,Caspase-3 activity was measured using a colorimetric assay kit,the measurement of intracellular ROS was based on the ROS-mediated conversion of non-fluorescent 2',7'-DCFH-DA into fluorescent DCFH,and Western bolt was used to detect the expression of PUMA.Secondly,the EC109 cells were treated with PTE (0,100μmol/L) for 24h after they were transfected with control or PUMA siRNA,then Western bolt was used to detect the expression of PUMA,CCK-8 was used to measure the cell viability.Results:Being treated with PTE (0,50,100,150μmol/L) for 12,24 and 36h,the cell viability of EC109 cells was decreased in a dose-and time-dependent manner(P＜0.01).After the treatment of PTE for 24h,The apoptotic index,Caspase-3 activity and ROS generation were increased (P＜0.01).Furthermore,when the expression of PUMA was decreased after siRNA transfection,the cytotoxic effects of PTE on EC109 cells decreased(P＜0.01).Conclusion:PTE treatment can effectively inhibit cell viability of EC109 cells,and the mechanism is related to the apoptosis induced by PUMA,PTE may be a potential new drug for treatment of esophageal cancer.

References:

［1］ Kamangar F,Dores GM,Anderson WF.Patterns of cancer incidence,mortality,and prevalence across five continents:defining priorities to reduce cancer disparities in different geographic regions of the world ［J］.J Clin Oncol,2006,24 (14):2137-2150.
［2］ Feng Y,Ke C,Tang Q,et al.Metformin promotes autophagy and apoptosis in esophageal squamous cell carcinoma by downregulating Stat3 signaling ［J］.Cell Death Dis,2014,5:e1088.
［3］ Schneider JG,Alosi JA,McDonald DE,et al.Effects of pterostilbene on melanoma alone and in synergy with inositol hexaphosphate ［J］.Am J Surg,2009,198 (5):679-684.
［4］ Yang Y,Yan X,Duan W,et al.Pterostilbene exerts antitumor activity via the Notch1 signaling pathway in human lung adenocarcinoma cells ［J］.PLoS One,2013,8 (5):e62652.
［5］ Nikhil K,Sharan S,Chakraborty A,et al.Role of isothiocyanate conjugate of pterostilbene on the inhibition of MCF-7 cell proliferation and tumor growth in Ehrlich ascitic cell induced tumor bearing mice ［J］.Exp Cell Res,2014,320 (2):311-328.
［6］ Lin VC,Tsai YC,Lin JN,et al.Activation of AMPK by pterostilbene suppresses lipogenesis and cell-cycle progression in p53 positive and negative human prostate cancer cells ［J］.J Agric Food Chem,2012,60 (25):6399-6407.
［7］ Siedlecka-Kroplewska K,Jozwik A,Boguslawski W,et al.Pterostilbene induces accumulation of autophagic vacuoles followed by cell death in HL60 human leukemia cells［J］.J Physiol Pharmacol,2013,64 (5):545-556.
［8］ Jeffers JR,Parganas E,Lee Y,et al.Puma is an essential mediator of p53-dependent and-independent apoptotic pathways ［J］.Cancer Cell,2003,4 (4):321-328.
［9］ 殷德涛,李红强,郑丽丽,等.PUMA在甲状腺乳头状癌中的表达及诱导细胞凋亡中的作用［J］.中华实验外科杂志,2012,29(2):230-232.
［10］ Pennathur A,Gibson MK,Jobe BA,et al.Oesophageal carcinoma ［J］.Lancet,2013,381 (9864):400-412.
［11］ Wu LF,Wei BL,Guo YT,et al.Apoptosis induced by adenosine involves endoplasmic reticulum stress in EC109 cells ［J］.Int J Mol Med,2012,30 (4):797-804.
［12］ Renaud S,Falcoz PE,Olland A,et al.Is radiofrequency ablation or stereotactic ablative radiotherapy the best treatment for radically treatable primary lung cancer unfit for surgery ［J］.Interact Cardiovasc Thorac Surg,2013,16 (1):68-73.
［13］ Zhang S,Yang Y,Liang Z,et al.Silybin-mediated inhibition of Notch signaling exerts antitumor activity in human hepatocellular carcinoma cells ［J］.PLoS One,2013,8 (12):e83699.
［14］ Park HJ,Kim MJ,Ha E,et al.Apoptotic effect of hesperidin through caspase3 activation in human colon cancer cells,SNU-C4 ［J］.Phytomedicine,2008,15 (1-2):147-151.
［15］ Mena S,Rodríguez ML,Ponsoda X,et al.Pterostilbene-induced tumor cytotoxicity:a lysosomal membrane permeabilization-dependent mechanism ［J］.PLoS One,2012,7 (9):e44524.
［16］ Nguyen KC,Willmore WG,Tayabali AF.Cadmium telluride quantum dots cause oxidative stress leading to extrinsic and intrinsic apoptosis in hepatocellular carcinoma HepG2 cells ［J］.Toxicology,2013,306:114-123.
［17］ Estaquier J,Vallette F,Vayssiere JL,et al.The mitochondrial pathways of apoptosis ［J］.Adv Exp Med Biol,2012,942:157-183.
［18］ Barillé-Nion S,Bah N,Véquaud E,et al.Regulation of cancer cell survival by BCL2 family members upon prolonged mitotic arrest:opportunities for anticancer therapy ［J］.Anticancer Res,2012,32 (10):4225-4233.
［19］ Wang Y,Zhang J,Wang Q,et al.Bryostatin 5 induces apoptosis in acute monocytic leukemia cells by activating PUMA and caspases ［J］.Eur J Pharmacol,2013,718 (1-3):340-349.
［20］ Choi AY,Choi JH,Hwang KY,et al.Licochalcone A induces apoptosis through endoplasmic reticulum stress via a phospholipase Cγ1-,Ca(2+)-,and reactive oxygen species-dependent pathway in HepG2 human hepatocellular carcinoma cells ［J］.Apoptosis,2014,19 (4):682-697.
［21］ Ma YC,Ke Y,Zi X,et al.Jaridonin,a novel ent-kaurene diterpenoid from Isodon rubescens,inducing apoptosis via production of reactive oxygen species in esophageal cancer cells ［J］.Curr Cancer Drug Targets,2013,13 (6):611-624.
［22］ Chang J,Rimando A,Pallas M,et al.Low-dose pterostilbene,but not resveratrol is a potent neuromodulator in aging and Alzheimers disease ［J］.Neurobiol Aging,2012,33 (9):2062-2071.

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国家自然科学基金资助项目（编号：81000938）
Last Update: 2015-02-02