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Establishment and evaluation of a mouse model for breast cancer lung metastasis with 4T1-luc

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
735-737
Research Field:
Publishing date:

Info

Title:
Establishment and evaluation of a mouse model for breast cancer lung metastasis with 4T1-luc
Author(s):
Gong Hongxia; Lin Hongsheng; Zhang Ying; Qi Xin
Oncology Department,Guang An Men Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China.
Keywords:
breast cancer; lung metastasis; animal model; luciferase; bioluminescence
PACS:
R73-3;R737.9
DOI:
10.3969/j.issn.1672-4992.2015.06.02
Abstract:
Objective:To establish the mouse model for breast cancer lung metastasis with luciferase-labeled 4T1 breast tumor cells.Methods:4T1-luc cells were injected into ten mouse tailsvein to establish a breast cancer lung metastasis model.In vivo bioluminescence imaging of tumor hypoxia dynamics wasused to follow tumor progresses.On 21th day,calculate thepulmonary metastasistubercle,and histological analysis using HE stain was applied to confirm the model.Results:Bioluminescence imaging of lung metastasis appeared on the fifth day.The pathological examinationrevealed that there were 100% mouse with lung metastasis.Histological analysis using HE stain confirmed the model.Conclusion:The mouse model forbreast cancer lung metastasis with 4T1-luc is successfully established.

References:

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Memo

Memo:
国家自然科学基金资助项目(编号：81273947)
Last Update: 2015-02-02