|Table of Contents|

Effects of glucosamine on proliferation and apoptosis of human HepG2 cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 05
Page:
601-604
Research Field:
Publishing date:

Info

Title:
Effects of glucosamine on proliferation and apoptosis of human HepG2 cells and its mechanism
Author(s):
Wu Weigang1; Liu Weilong1; Zhuge Fuyan2; Tian Yuan1; Liu Zhuobing1; Chen Xinchun1; Zhou Boping1
1.Key Laboratory of Infection and Immunity，Institute of Hepatology，Shenzhen Third People's Hospital Affiliated To Guangdong Medical College,Guangdong Shenzhen 518112,China;2.College of Biophotonics，South China Normal University，Guangdong Guangzhou 510631，China.
Keywords:
glucosamine; hepatoma cells; HepG2; proliferation; apoptosis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2015.05.07
Abstract:
Objective：To study the effects of glucosamine on the proliferation and apoptosis of human HepG2 cells，and its possible molecular mechanism.Methods:The proliferation of human hepatoma HepG2 cells was detected by CCK-8 method.Cell cycle and apoptosis were detected by flow cytometry.The expression level of ERK and P-ERK proteins were detected by Western blot.Results:Glucosamine can inhibit the proliferation of human hepatoma HepG2 cells，and the inhibition rate enhanced with the increase of drug concentration.At the same time，treatment with glucosamine of 2.0mmol/L and 4.0mmol/L for 72 hours may induce apoptosis of human hepatoma HepG2.In addition，with the increase of drug concentration，and the phosphorylation level of ERK protein of human hepatoma HepG2 cells decreased gradually.Conclusion:Glucosamine may inhibit the proliferation of human hepatoma HepG2 cells and induce its apoptosis through the phosphorylation level of ERK protein and affect the cell cycle of HepG2 cells.

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Memo

Memo:
国家自然科学基金面上项目（编号：81372227）；深圳市科技计划项目（编号：CXZZ20130515163643)
Last Update: 2015-01-30