|Table of Contents|

Targeted suppression of miR-19a enhanced chemosensitivity of cervical cancer HeLa cells to cisplatin

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 05
Page:
604-607
Research Field:
Publishing date:

Info

Title:
Targeted suppression of miR-19a enhanced chemosensitivity of cervical cancer HeLa cells to cisplatin
Author(s):
Cui Lichun; Du Jianfei; Geng Huisheng; Han Liangfu; Liu Jinpeng
Cancer Center，Chang'an Hospital，Shaanxi Xi'an 710018,China.
Keywords:
cervical cancer; chemosensitivity; miR-19a; PTEN; AKT signaling pathway
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2015.05.08
Abstract:
Objective：To investigate the effect of miR-19a antisense oligonucleotide （miR-19a-ASO） on cisplatin chemosensitivity in human HeLa cells and its underlying mechanism.Methods：miR-19a-ASO was transfected to HeLa cells using Lipofectamine 2000.Real-time PCR was used to evaluate the miR-19a expression.Cell viability was measured by methylthiazolyltetrazolium （MTT） assay.The expression of PTEN，AKT，and p-AKT(Ser473） protein was detected by Western blot.Results：miR-19a-ASO inhibited the expression of miR-19a effectively.HeLa cells transfected with miR-19a-ASO was sensitized to cisplatin （P<0.05） by MTT assay.Targeting to miR-19a could up-regulate PTEN，and down-regulate p-AKT（Ser473） expression.Conclusion：Silence of miR-19a increase cell sensitivity to cisplatin probably by suppressing AKT signaling pathway，providing a new target for cervical cancer therapy.

References:

［1］Jemal A，Bray F，Center MM，et al.Global cancer statistics［J］.CA Cancer J Clin，2011，61(2)：69-90.
［2］Bartel DP.MicroRNAs：genomics，biogenesis，mechanism，and function［J］.Cell，2004，116(2)：281-297.
［3］Ju J，Jiang J，Fesler A.miRNA：the new frontier in cancer medicine［J］.Future Med Chem，2013，5(9)：983-985.
［4］Liang Z，Li Y，Huang K，et al.Regulation of miR-19 to breast cancer chemoresistance through targeting PTEN［J］.Pharm Res，2011，28(12)：3091-3100.
［5］Calin GA，Liu CG，Sevignani C，et al.MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias［J］.Proc Natl Acad Sci USA，2004，101(32)：11755-11760.
［6］Xu XM，Wang XB，Chen MM，et al.MicroRNA-19a and-19b regulate cervical carcinoma cell proliferation and invasion by targeting CUL5［J］.Cancer Lett，2012，322(2)：148-158.
［7］Gocze K，Gombos K，Juhasz K，et al.Unique microRNA expression profiles in cervical cancer［J］.Anticancer Res，2013，33(6)：2561-2567.
［8］Hu X，Schwarz JK，Lewis JS，et al.A microRNA expression signature for cervical cancer prognosis［J］.Cancer Res，2010，70(4)：1441-1448.
［9］Ma D，Zhang YY，Guo YL，et al.Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer［J］.Chin Med J，2012，125(23)：4270-4276.
［10］Doebele C，Bonauer A，Fischer A，et al.Members of the microRNA-17-92 cluster exhibit a cell-intrinsic antiangiogenic function in endothelial cells［J］.Blood，2010，115(23)：4944-4950.
［11］Bonauer A,Dimmeler S.The microRNA-17-92 cluster：still a miRacle［J］?Cell Cycle，2009，8(23)：3866-3873.
［12］Kurokawa K，Tanahashi T，Iima T，et al.Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells［J］.J Gastroenterol，2012，47(8)：883-895.
［13］Yin R，Bao W，Xing Y，et al.MiR-19b-1 inhibits angiogenesis by blocking cell cycle progression of endothelial cells［J］.Biochem Biophys Res Commun，2012，417(2)：771-776.
［14］陈诗书,戴冰冰.基因治疗的研究现状与评价［J］.中华肿瘤杂志，2002，24(4)：3.
［15］Olie RA，Simoes-Wust AP，Baumann B，et al.A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung cancer cells to chemotherapy［J］.Cancer Res，2000，60(11)：2805-2809.
［16］高晓康，李青，王禾,等.反义survivin基因对786-O细胞的体外作用及其对表阿霉素诱导细胞凋亡作用的观察［J］.中华肿瘤杂志，2005，27(8)：3.
［17］范幸，王比男，李建军.PTEN影响宫颈癌HeLa细胞对顺铂的化疗敏感性［J］.中日友好医院学报，2012，26(1)：25-28,38.
［18］Han Z，Hong L，Han Y，et al.Phospho Akt mediates multidrug resistance of gastric cancer cells through regulation of P-gp，Bcl-2 and Bax［J］.J Exp Clin Cancer Res，2007，26(2)：261-268.
［19］O'gorman DM，Mckenna SL，Mcgahon AJ，et al.Inhibition of PI3-kinase sensitises HL60 human leukaemia cells to both chemotherapeutic drug-and Fas-induced apoptosis by a JNK independent pathway［J］.Leuk Res，2001，25(9)：801-811.

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Last Update: 2015-01-30