|Table of Contents|

Effect of eIF5A1 gene on chemosensitivity of human NSCLC cell lines

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 05
Page:
608-613
Research Field:
Publishing date:

Info

Title:
Effect of eIF5A1 gene on chemosensitivity of human NSCLC cell lines
Author(s):
Li Yanqi1; Li Qin2; He Lin2; Hui Linping2; Xu Chong'an1
1.Department of Oncology Medicine，the Fourth Affiliated Hospital of China Medical University，Liaoning Shenyang 110032，China；2.Central Laboratory，the Fourth Affiliated Hospital of China Medical University，Liaoning Shenyang 110032，China.
Keywords:
NSCLC; eIF5A1; chemosensitivity
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2015.05.09
Abstract:
Objective：To investigate the effect of eIF5A1 gene on chemosensitivity of human NSCLC cell lines and the possible mechanism.Methods：Expression levels of eIF5A1 mRNA in different NSCLC cell lines were measured by RT-PCR .The sensitivities to gemcitabine for cells were detected by MTT assay.The correlation between the above two was analysized.The eukaryotic expression plasmid pEGFP-C1-eIF5A1 was constructed and transfected into the LTEP-a-2 cells by liposome transfection reagent.The transfection was proved effective by the fluorescence microscopy，RT-PCR and Western blot.MTT assay was used to investigate the effects of eIF5A1 overexpression on the drug sensitivities of LTEP-a-2 cells.The change of apoptosis rate and cell cycle were detected by flow cytometry （FCM).Results：Lung squamous cell lines had higher eIF5A1 expression levels and sensitvities to gemcitabine than adencarcinoma cell lines （P<0.001）.eIF5A1 mRNA and protein expression levels of LTEP-a-2 cell line with the lowest eIF5A1 expression level were significantly increased after transfection with pEGFP-C1-eIF5A1 （P<0.001）.Compaired with the empty vector group or un-transfected group，the sestivities to cisplatin，gemcitabine，paclitaxel，docetaxel were significantly increased for eIF5A1 gene transfected group （P<0.001），inforced eIF5A1 expression increased the cell cycle arrest in S and G2/M phase （P<0.001）.With the treatment of chemotherapeutic agents，the cell apoptosis also significantly increased in the eIF5A1 gene transfected group （P<0.001）.Conclusion：Overexpression of eIF5A1 in non-small cell lung cancer may increase the sensitivity of cells to chemotherapeutic agents.eIF5A1 may influence chemosensitivity of non-small cell lung cancer through promoting apoptosis-accelerating effects of chemotherapeutic agents and changing the cell cycle.

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Last Update: 2015-01-30