|Table of Contents|

Expression and clinical significance of the urine exosomes in urothelial cell carcinoma of the bladder

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 06
Page:
1090-1093
Research Field:
Publishing date:

Info

Title:
Expression and clinical significance of the urine exosomes in urothelial cell carcinoma of the bladder
Author(s):
LIU Liangcheng; CAO Guihua; TENG Donghai; LI Tiejun; DU Jianping; LI Wei; LI Qiang; HUANG Guimin; GUO Jianjun
Department of Urology,People's Hospital of Leshan,Sichuan Leshan 614000，China.
Keywords:
urothelial cell carcinoma of the bladder; urine; exosome; biomarker
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2023.06.020
Abstract:
Objective:To explore the expression of urine exosomes in urothelial cell carcinoma of the bladder and its clinical relevance.Methods:Tumour-derived exosomes in urine samples of 30 urothelial cell carcinoma of the bladder patients and 15 healthy control peoples were separated using ultracentrifugation.Morphologic characteristics of exosomes were observed by the electron microscope and protein concentration was determined by the BCA protein assay kit.The expression of CD9 in exosomes was detected by Western blot.Results:The level of urine exosomes in healthy control people was significantly different from that in experimental group ［(125.99±47.71)μg/μL vs (259.74±57.47)μg/μL,P＜0.000 1］.According to the degree of tumor invasion,there was a statistically significant difference in urine exosome level between patients with non-muscle invasive bladder cancer and patients with muscle-invasive bladder cancer.However,muscle-invasive bladder cancer patients were no significant differences between subgroups.Conclusion:Detection of urine exosome may offer a convenient tool for early diagnosis of urothelial cell carcinoma of the bladder.

References:

［1］BABJUK M,BURGER M,CAPOUN O,et al.European association of urology guidelines on non-muscle-invasive bladder cancer (Ta,T1,and carcinoma in situ)［J］.Eur Urol，2022，81(1):75-94.
［2］LOKESHWAR VB,SOLOWAY MS.Current bladder tumor tests:does their projected utility fulfill clinical necessity［J］.J Urol，2001，165(4):1067-1077.
［3］YOSHIDA K,TSUDA M,MATSUMOTO R,et al.Exosomes containing ErbB2/CRK induce vascular growth in premetastatic niches and promote metastasis of bladder cancer［J］.Cancer Sci，2019，110(7):2119-2132.
［4］MATSUZAKI K,FUJITA K,JINGUSHI K,et al.MiR-21-5p in urinary extracellular vesicles is a novel biomarker of urothelial carcinoma［J］.Oncotarget，2017，8(15):24668-24678.
［5］ZHENG R,DU M,WANG X,et al.Exosome-transmitted long non-coding RNA PTENP1 suppresses bladder cancer progression［J］.Mol Cancer，2018，17(1):143.
［6］LI W,LI C,ZHOU T,et al.Role of exosomal proteins in cancer diagnosis［J］.Mol Cancer，2017，16(1):145.
［7］STREET JM,KORITZINSKY EH,GLISPIE DM,et al.Urine exosome isolation and characterization［J］.Methods Mol Biol，2017，1641:413-423.
［8］THRY C,ZITVOGEL L,AMIGORENA S.Exosomes:composition,biogenesis and function［J］.Nat Rev Immunol，2002，2(8):569-579.
［9］KOWAL J,ARRAS G,COLOMBO M,et al.Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes［J］.Proc Natl Acad Sci USA，2016，113(8):E968-E977.
［10］MELO SA,LUECKE LB,KAHLERT C,et al.Glypican-1 identifies cancer exosomes and detects early pancreatic cancer［J］.Nature，2015，523(7559):177-182.
［11］THONGBOONKERD V.Practical points in urinary proteomics［J］.J Proteome Res，2007，6(10):3881-3890.
［12］MENG Y,SUN J,WANG X,et al.Exosomes:A promising avenue for the diagnosis of breast cancer［J］.Technol Cancer Res Treat，2019，18:1533033818821421.
［13］CHEN W,JIANG J,XIA W,et al.Tumor-related exosomes contribute to tumor-promoting microenvironment:An immunological perspective［J］.J Immunol Res，2017，2017:1073947.
［14］NAKAMURA K,SAWADA K,KINOSE Y,et al.Exosomes promote ovarian cancer cell invasion through transfer of CD44 to peritoneal mesothelial cells［J］.Mol Cancer Res，2017，15(1):78-92.
［15］LOPEZ-BELTRAN A,CHENG L,GEVAERT T,et al.Current and emerging bladder cancer biomarkers with an emphasis on urine biomarkers［J］.Expert Rev Mol Diagn，2020，20(2):231-243.
［16］LING X,ZHANG G,XIA Y,et al.Exosomes from human urine-derived stem cells enhanced neurogenesis via miR-26a/HDAC6 axis after ischaemic stroke［J］.J Cell Mol Med，2020，24(1):640-654.
［17］TOTH B,NIEUWLAND R,LIEBHARDT S,et al.Circulating microparticles in breast cancer patients:a comparative analysis with established biomarkers［J］.Anticancer Res，2008，28(2A):1107-1112.
［18］ELSHARKAWI F,ELSABAH M,SHABAYEK M,et al.Urine and serum exosomes as novel biomarkers in detection of bladder cancer［J］.Asian Pacific Journal of Cancer Prevention,2019,20(7):2219.
［19］ZHANG B,YIN Y,LAI RC,et al.Immunotherapeutic potential of extracellular vesicles［J］.Front Immunol，2014，5:518.
［20］FRANZEN CA,BLACKWELL RH,TODOROVIC V,et al.Urothelial cells undergo epithelial-to-mesenchymal transition after exposure to muscle invasive bladder cancer exosomes［J］.Oncogenesis，2015，4(8):e163.
［21］WEE I,SYN N,SETHI G,et al.Role of tumor-derived exosomes in cancer metastasis［J］.Biochim Biophys Acta Rev Cancer，2019，1871(1):12-19.
［22］XU Y,LOU J,YU M,et al.Urinary exosomes diagnosis of urological tumors:A systematic review and meta-analysis［J］.Front Oncol，2021，11:734587.

Memo

Memo:
四川省卫计委课题基金项目（编号：17PJ131）；四川省青年科技基金项目（编号：17QNJJ0001）
Last Update: 1900-01-01