|Table of Contents|

Clinical application value of plasma Septin9 DNA methylation combined with FOBT detection in patients with colorectal cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 06
Page:
1085-1090
Research Field:
Publishing date:

Info

Title:
Clinical application value of plasma Septin9 DNA methylation combined with FOBT detection in patients with colorectal cancer
Author(s):
LI Na1; HUI Lingyun1; FENG Ai1; ZHANG Lin1; WANG Yawen1; 2
1.Department of Clinical Laboratory；2.Biobank，the First Affiliated Hospital of Xi'an Jiaotong University，Shaanxi Xi'an 710061，China.
Keywords:
colorectal cancer; benign adenoma; fecal occult blood test; Septin9 DNA methylation
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2023.06.019
Abstract:
Objective:To investigate the application value of plasma Septin9 DNA methylation and fecal occult blood test (FOBT) in the diagnosis of colorectal cancer.Methods:The clinical data of 101 patients with colorectal benign adenoma and 209 patients with colorectal cancer who were diagnosed by pathological examination in our hospital from June 2017 to January 2022 were retrospectively collected as the benign adenoma group and the colorectal cancer group,respectively.The physical examination data of 98 healthy people who underwent physical examination in our hospital in the same period were selected as the normal control group,and the general data,plasma Septin9 DNA methylation and FOBT positive status of the three groups were compared.The plasma Septin9 DNA methylation and FOBT positive status of colorectal cancer patients with different pathological features and clinical stages were compared.The receiver operating curve (ROC) was used to assess the diagnostic value of plasma Septin9 DNA methylation and FOBT tests alone and in combination for colorectal cancer.Results:Compared with the three groups,the positive rates of plasma Septin9 DNA methylation and FOBT in the colorectal cancer group were higher than those in the benign adenoma group and the normal control group,and those in the benign adenoma group were higher than those in the normal control group (P<0.05).The positive rates of plasma Septin9 DNA methylation and FOBT in colorectal cancer patients with low tumor differentiation,lymph node metastasis and vascular/nerve invasion were higher than those in CRC patients with high tumor differentiation,moderate tumor differentiation,and CRC patients without lymph node metastasis and vascular/nerve invasion (P<0.05).With the increase of TNM clinical stage of colorectal cancer,the positive rate of plasma Septin9 DNA methylation and FOBT increased,and the difference between groups was statistically significant (P<0.05).The sensitivity and area under curve (AUC) of Septin9 DNA methylation combined with FOBT in the diagnosis of colorectal cancer (87.08%,0.885) were higher than those of Septin9 DNA methylation and FOBT alone (59.33%,0.744 and 52.63%,0.643,P<0.05).The CRC specificity,positive predictive value and negative predictive value of Septin9 DNA methylation diagnosis were 89.45%,85.52% and 67.68%respectively.The FOBT values were 76.38%,70.06% and 60.56% respectively,and the combined diagnosis values were 89.95%,90.10% and 86.89% respectively.The combined diagnosis was superior to the single diagnosis.Conclusion:With the increase of the malignant degree of colorectal lesions,the positive rate of plasma Septin9 DNA methylation and FOBT increased.The combination of plasma Septin9 DNA methylation and FOBT has a high diagnostic value for colorectal cancer,and can be used as a laboratory diagnostic index for colorectal cancer.

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Memo

Memo:
西安交通大学第一附属医院临床研究课题（编号：XJTU1AF-CRF-2017-014）
Last Update: 1900-01-01