|Table of Contents|

CTNND1 regulates pancreatic cancer cell proliferation,migration and invasion through the Wnt/β-catenin signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 05
Page:
818-825
Research Field:
Publishing date:

Info

Title:
CTNND1 regulates pancreatic cancer cell proliferation,migration and invasion through the Wnt/β-catenin signaling pathway
Author(s):
HUANG Xiaobin1; 2; 3; XIE Mengyi4; LIU Xingyu2; HUANG Xiaodong2; LI Jiayu5; LAN Chuan1; DENG Dawei1; ZHANG Guangnian1; LI Yong1; LI Jianshui1
1.Department of Hepatobiliary Surgery,Affiliated Hospital of North Sichuan Medical College,Sichuan Nanchong 637000,China;2.North Sichuan Medical College,Sichuan Nanchong 637000,China;3.Zigong Hospital of Woman and Children Healthcare,Sichuan Zigong 634000,China;4.Institute of Hepatobiliary Pancreatic Intestine,Affiliated Hospital of North Sichuan Medical College,Sichuan Nanchong 637000,China;5.School of Basic Medicine,Naval Medical University,Shanghai 200433,China.
Keywords:
pancreatic cancer; CTNND1; Wnt/β-Catenin; cell migration; tumor invasion; cell proliferation
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2024.05.006
Abstract:
Objective:To study the knockdown of CTNND1 through Wnt/ β-Catenin signaling pathway regulates the proliferation,migration and invasion of pancreatic cancer cells,and further provides theoretical basis for molecular targeted therapy in pancreatic cancer.Methods:The expression of CTNND1 in pancreatic cancer and normal tissues was validated through bioinformatics analysis,and further confirmed by immunohistochemistry(IHC) and qPCR.Transwell,wound healing,and cell proliferation assays were conducted to investigate the impact of CTNND1 on the proliferation,migration,and invasion of pancreatic cancer cells.Subcutaneous tumor formation experiments in nude mice were performed to assess the tumorigenic ability of human pancreatic cancer cells with altered CTNND1 expression.Results:Knockdown of CTNND1 in pancreatic cancer cells inhibited the Wnt/β-catenin signaling pathway as well as the proliferation,migration,and invasion capabilities of these cells.Adding of LiCl(a specific activator of Wnt/β-catenin) partially restored the proliferation,migration,and invasion capabilities of CTNND1-knockdown pancreatic cancer cells.Subcutaneous tumor formation in nude mice demonstrated that CTNND1 knockdown suppressed tumor growth.Conclusion:Knockdown of CTNND1 can regulate the proliferation,migration,invasion,and subcutaneous tumor formation ability of pancreatic cancer through the Wnt/β-catenin signaling pathway,both in vitro and in vivo.

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Memo

Memo:
2018四川省科学技术局项目(编号：2018JY0489);2019四川省南充市市校科技战略合作专项(编号：19SXHZ0175)；2020四川省卫生和计划生育委员会研究项目(编号：20PJ150)；2021四川省卫生和计划生育委员会研究项目(编号：21PJ104)
Last Update: 2024-01-30