|Table of Contents|

Impact of Taxifolin on the malignant progression of acute lymphoblastic leukemia cells by regulating the MCP-1/CCR2 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 05
Page:
799-804
Research Field:
Publishing date:

Info

Title:
Impact of Taxifolin on the malignant progression of acute lymphoblastic leukemia cells by regulating the MCP-1/CCR2 axis
Author(s):
WANG Ruijuan; LI Chao; DUAN Lijuan; QIN Fan; FENG Jinliang
Department of Hematology,Nanyang Central Hospital,Henan Nanyang 473000,China
Keywords:
Taxifolin; CCL2/CCR2 axis; acute lymphocytic leukemia cells; malignant progression
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2024.05.003
Abstract:
Objective:To investigate the impact of Taxifolin on the malignant progression of acute lymphoblastic leukemia(ALL) cells and the role of monocyte chemotactic protein-1(CCL2)/CC chemokine receptor 2(CCR2) axis.Methods:ALL-T lymphocytes CCRF-CEM were cultured in vitro.CCK-8 method was applied to detect the effects of different concentrations of Taxifolin on the viability of CCRF-CEM cells,and the concentration of Taxifolin was selected for this experiment.CCRF-CEM cells were divided into control group,Taxifolin-L group,Taxifolin-M group,Taxifolin-H group,and Taxifolin+rCCL2 group.EDU method was applied to detect the proliferation of CCRF-CEM cells in each group.Flow cytometry was applied to detect apoptosis of CCRF-CEM cells in each group.Transwell was applied to detect the invasiveness of CCRF-CEM cells in each group.Plate cloning experiment was applied to detect the colony forming ability of CCRF-CEM cells in each group.RT-PCR was applied to detect the expression levels of CCL2 and CCR2 genes in CCRF-CEM cells in each group and Western blot was applied to detect the expression levels of CCL2,CCR2,Bcl-2,Bax,and Caspase-3 proteins in CCRF-CEM cells in each group.Results:25,50 and 100 μmol/L Taxifolin were selected for subsequent experiments.Compared with the control group,the apoptosis rate of CCRF-CEM cells,and the protein expression levels of Bax and Caspase-3 in Taxifolin-L,M,and H groups were obviously higher,the EDU positive cell rate of CCRF-CEM cells,number of invading cells,number of cell clones,expression levels of CCL2 and CCR2 genes,and expression levels of CCL2,CCR2,and Bcl-2 proteins in CCRF-CEM cells were obviously lower,and it was dose-dependent(P＜0.05).Compared with the Taxifolin-H group,the apoptosis rate of CCRF-CEM cells,and the protein expression levels of Bax and Caspase-3 in Taxifolin+rCCL2 groups were obviously lower,the EDU positive cell rate of CCRF-CEM cells,number of invading cells,number of cell clones,expression levels of CCL2 and CCR2 genes,and expression levels of CCL2,CCR2,and Bcl-2 proteins were obviously higher(P＜0.05).Conclusion:Taxifolin may inhibit the malignant progression of CCRF-CEM cells by inhibiting the CCL2/CCR2 axis.

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Memo

Memo:
河南省医学科技攻关联合共建项目(编号：LHGJ202203462)
Last Update: 2024-01-30