|Table of Contents|

Mechanism of rhamnetin inhibiting malignant biological behaviors of hepatocellular carcinoma cells by down-regulating NEK2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 05
Page:
792-799
Research Field:
Publishing date:

Info

Title:
Mechanism of rhamnetin inhibiting malignant biological behaviors of hepatocellular carcinoma cells by down-regulating NEK2
Author(s):
ZHANG Lin1; XU Houping2; LIU Daiqun1; WANG Sijia1; LI Guangxia1; GAO Li1
1.The Affiliated TCM Hospital of Southwest Medical University General Medicine Department,Sichuan Luzhou 646000,China;2.The Affiliated TCM Hospital of Southwest Medical University Prevention and Treatment Center,Sichuan Luzhou 646000,China.
Keywords:
rhamnetin; hepatocellular carcinoma cell; proliferation; invasion
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.05.002
Abstract:
Objective:To analyze the mechanism of rhamnetin inhibiting the proliferation,migration and invasion of hepatocellular carcinoma(HCC) cells by NIMA-related kinase 2(NEK2).Methods:The possible target(NEK2) of rhamnetin was analyzed by network pharmacology.According to TCGA_LIHC database,the expression of NEK2 in HCC tissues and its relationship with prognosis were analyzed.The effects of different concentrations of rhamnetin on the proliferation of HCC cells and expression of NEK2 protein were detected.The control group and rhamnetin group were set up.The cells proliferation,migration and invasion were detected.The effect of rhamnetin on the volume and weight of xenograft tumors,and expression of Ki67 protein were observed.The shNEK cells and stable overexpression NEK2 cells were constructed to observe the effects of rhamnetin on biological behaviors of HCC cells by inhibiting NEK2.Results:Compared with para-cancerous tissues,the expression of NEK2 in HCC tissues was up-regulated(P＜0.05).With the increase of rhamnetin concentration,relative survival rates of HepG2 and Hep3B were decreased(P＜0.05).The proliferation activity,number of invasion and migration cells in rhamnetin group were lower than those in control group(P＜0.05),volume and weight of xenograft tumors were lower than those in control group(P＜0.05),and the expression of Ki67 protein was significantly lower than that in control group(P＜0.05).With the increase of rhamnetin concentration and action time,expression of NEK2 protein was significantly decreased(P＜0.05).Silencing NEK2 gene could promote cells proliferation,invasion and migration,while NEK2 overexpression could inhibit cells proliferation,invasion and migration(P＜0.05).Conclusion:Rhamnetin can inhibit the expression of NEK2 protein,and inhibit the proliferation,migration and invasion of HCC cells.

References:

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国家重点研发计划项目(编号：2018YFC1704701)
Last Update: 2024-01-30