|Table of Contents|

The expression of PIM1 in diffuse large B-cell lymphoma tissue with co-expression of C-MYC/BCL2 and its relationship with clinicopathological characteristics

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 13
Page:
2491-2496
Research Field:
Publishing date:

Info

Title:
The expression of PIM1 in diffuse large B-cell lymphoma tissue with co-expression of C-MYC/BCL2 and its relationship with clinicopathological characteristics
Author(s):
XU Ruihuan; XU Juan; YANG Wenxiu
Department of Pathology，the Affiliated Hospital of Guizhou Medical University，Guizhou Guiyang 550001，China.
Keywords:
double-expression lymphoma (DEL); diffuse large B-cell lymphoma; Hans classification; PIM1; p65; prognosis
PACS:
R733.4
DOI:
10.3969/j.issn.1672-4992.2023.13.022
Abstract:
Objective：To detect the mutation and expression of PIM1 gene in C-MYC/BCL2 double-expression diffuse large B-cell lymphoma (DEL),and to explore the relationship between PIM1 expression and clinical pathological features of DEL.Methods：Clinical cases diagnosed as DLBCL by the Department of Pathology of our hospital from January 1,2010 to December 31,2018 were collected.Immunohistochemistry (IHC) staining was used to detect the expression of C-MYC and BCL2 proteins.Qualified cases were selected for detecting the expression of p65.sanger sequencing were used to detect PIM1 mutations,and RT-qPCR was used to measure the expression of PIM1 mRNA.Cases were followed up and appropriate statistical methods were used for statistical analysis.Results：PIM1 gene mutation in DEL cases and its effect on PIM1 expression:39 DEL cases were included，the total mutation rate of PIM1 in DEL was 56.41% (22/39).The expression of PIM1 mRNA in the cases with/without PIM1 mutation was statistically different (P=0.012),and it was significantly increased in the PIM1 mutation group.NF-κB (p65) protein expression:The total positive expression rate was 53.85% (21/39),and respectively in non-GCB type DEL (16/23) was significantly higher than that in GCB type DEL (5/16) (P=0.025).The relationship between PIM1 expression and clinicopathological characteristics of DEL:The expression of PIM1 mRNA was also related to Ann Arbor staging and serum LDH level.It is significantly increased in late clinical stage or DEL cases with abnormally elevated serum LDH (P=0.046,P=0.048).The effect of PIM1,p65 on the expression of C-MYC and BCL2 in DEL:The expression of C-MYC protein in the p65 positive was statistically significantly increased compared with negative expression group(P=0.021).In DEL cases,the expression of C-MYC and BCL2 has relationship with PIM1 mRNA，PIM1 mRNA significantly increased with high expression（P=0.015,0.039）.Conclusion：In DEL,there are high-frequency mutations in the PIM1 gene and up-regulation of RNA expression,the up-regulation of RNA expression of PIM1 mRNA may be related to its mutation,and abnormal PIM1 may be related to the poor prognostic characteristics of DEL.p65 influence C-MYC protein expression in DEL,not related to abnormal PIM1.

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Memo

Memo:
贵州省贵阳市科技计划项目［编号：筑科合同（2019）9-1-6号］
Last Update: 2023-05-31