|Table of Contents|

Expression of ADAM15,MMP-2 and MMP-9 mRNA and the significance in breast cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 06
Page:
1056-1060
Research Field:
Publishing date:

Info

Title:
Expression of ADAM15,MMP-2 and MMP-9 mRNA and the significance in breast cancer
Author(s):
OU Xiaobo1; WU Minhua2; ZHOU Mi3; CHEN Mei2; JIN Junfeng1
1.Department of Pathology，Zhuhai Campus of Zunyi Medical University，Guangdong Zhuhai 519041，China；2.School of Basic Medicine,Guangdong Medical University,Guangdong Zhanjiang 524023，China;3.Department of Clinical Medicine，Guangdong Zhuhai Campus of Zunyi Medical University，Guangdong Zhuhai 519041，China.
Keywords:
breast neoplasms; ADAM15; MMP-2; MMP-9; tumor metastasis; bioinformatics
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.06.014
Abstract:
Objective：To explore the expression of ADAM15,MMP-2 and MMP-9 mRNA in breast cancer,relationship with clinicopathological features and effects on breast cancer prognosis.Methods：The expression of ADAM15,MMP-2,and MMP-9 mRNA was measured in 45 breast cancers and 20 benign breast lesions by in situ hybridization.The relationship between ADAM15 mRNA and the clinicopathological features of breast cancer and the correlation between ADAM15 and MMP-2 and MMP-9 mRNA expression were analyzed.Breast cancer transcriptome data were obtained from the TCGA database to verify the relationship between ADAM15,MMP-2,and MMP-9 mRNA and the clinicopathological features of breast cancer by bioinformatic analysis.Results：ADAM15 mRNA is highly expressed in breast cancer.The high expression was closely associated with lymphatic metastasis and clinical stage,but was not associated with age and tumor size.Compared with benign breast lesions,positive rate of MMP-2 mRNA and MMP-9 mRNA in breast cancer wsa significantly increased.The high expression was associated with lymphatic metastasis.The expression of ADAM15 mRNA was positively correlated with MMP-2 and MMP-9 mRNA.The bioinformatics analysis showed ADAM15 mRNA was highly expressed in breast cancer and high expression of ADAM15 and the expression was negatively correlated with prognosis.Conclusion：ADAM15 mRNA was highly expressed in breast cancer.The expression was associated with lymph node metastasis and inversely correlated with the prognosis of the patients.ADAM15 mRNA was likely to promote breast cancer metastasis through upregulating the expression of MMP-2 and MMP-9 mRNA.

References:

［1］SIEGEL RL，MILLER KD，JEMAL A.Cancer statistics，2020［J］.CA Cancer J Clin，2020，870(1)：7-30.
［2］雷少元，郑荣寿，张思维，等.中国2015年分省份乳腺癌死亡发病比分析［J］.肿瘤预防与治疗，2021，34(07)：591-596. LEI SY，ZHENG RS，ZHANG SW，et al.Analysis of the death and the incidence of breast cancer in China in 2015［J］.Journal of Cancer Control and Treatment，2021，34(07)：591-596.
［3］金婷，任小莉，杨巧，等.高表达DNAJA1促进乳腺癌细胞增殖及侵袭且与患者的不良预后及化疗抵抗密切相关［J］.现代肿瘤医学，2022，30(11)：1983-1989. JIN T，REN XL，YANG Q，et al.High expression of DNAJA1 promotes breast cancer cell proliferation and invasion and is closely related to poor prognosis and chemotherapy resistance in patients［J］.Modern Oncology，2022，30(11)：1983-1989.
［4］CHANG JM，LEUNG J，MOY L，et al.Axillary nodal evaluation in breast cancer:State of the art［J］.Radiology，2020，295(3)：500-515.
［5］WANG X，ROJAS-QUINTERO J，ZHANG D，et al.A disintegrin and metalloproteinase domain-15 deficiency leads to exaggerated cigarette smoke-induced chronic obstructive pulmonary disease(COPD)-like disease in mice ［J］.Mucosal Immunol，2021，14(2)：342-356.
［6］JANCZI T，MEIER F，FEHRL Y，et al.A novel pro-inflammatory mechanosensing pathway orchestrated by the disintegrin metalloproteinase ADAM15 in synovial fibroblasts［J］.Cells，2021，10(10)：2705.
［7］MATTERN J，ROGHI CS，HURTZ M，et al.ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells［J］.Sci Rep，2019，9(1)：12540.
［8］YU C，WANG Y，LIU T，et al.The microRNA miR-3174 suppresses the expression of ADAM15 and inhibits the proliferation of patient-derived bladder cancer cells［J］.Onco Targets Ther，2020，13：4157-4168.
［9］LIN Q，JIA Y，ZHANG D，et al.NCK1-AS1 promotes the progression of melanoma by accelerating cell proliferation and migration via targeting miR-526b-5p/ADAM15 axis［J］.Cancer Cell Int，2021，21(1)：367.
［10］BACOLOD MD，BARANY F.A unified transcriptional,pharmacogenomic,and gene dependency approach to decipher the biology，diagnostic markers，and therapeutic targets associated with prostate cancer metastasis［J］.Cancers (Basel)，2021，13(20)：5158.
［11］XU JH，GUAN YJ，ZHANG YC，et al.ADAM15 correlates with prognosis,immune infiltration and apoptosis in hepatocellular carcinoma［J］.Aging (Albany NY)，2021，13(16)：20395-20417.
［12］JANA S，CHUTE M，HU M，et al.ADAM (a disintegrin and metalloproteinase) 15 deficiency exacerbates Ang II (angiotensin II)-induced aortic remodeling leading to abdominal aortic aneurysm［J］.Arterioscler Thromb Vasc Biol，2020，40(8)：1918-1934.
［13］ZHOU J，WANG A，CAI T，et al.Integrin α3/α6 and αV are implicated in ADAM15-activated FAK and EGFR signalling pathway individually and promote non-small-cell lung cancer progression［J］.Cell Death Dis，2022，13(5)：486.
［14］DAS S，DE S，SENGUPTA S.Post-transcriptional regulation of MMP2 mRNA by its interaction with miR-20a and nucleolin in breast cancer cell lines［J］.Mol Biol Rep，2021，48(3)：2315-2324.
［15］SHAKERY T，SAFARI F.Downregulation of pinkbar/pAKT and MMP2/MMP9 expression in MDA-MB-231 breast cancer cells as potential targets in cancer therapy by hAMSCs secretome［J/OL］.Cells Tissues Organs［2022-09-15］.https://pubmed.ncbi.nlm.nih.gov/34695828/doi:10.1159/000520370.
［16］OGAWA K，LIN Q，LI L，et al.Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway［J］.J Hematol Oncol，2019，12(1)：144.

Memo

Memo:
贵州省科学技术基金资助项目(编号：黔科合 J 字 LKZ［2011］40 号)；广东省基础与应用基础研究项目(编号： 2019A1515012007)；广东省教育厅科研项目（编号：2019KTSCX048）
Last Update: 1900-01-01