|Table of Contents|

Cinobufotalin-mediated miR-497-5p/VEGFA pathway regulates proliferation,apoptosis and angiogenesis of lung cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 04
Page:
632-637
Research Field:
Publishing date:

Info

Title:
Cinobufotalin-mediated miR-497-5p/VEGFA pathway regulates proliferation,apoptosis and angiogenesis of lung cancer cells
Author(s):
CAI Yuliang1 ; ZHENG Jie2 ; ZHANG Jinhua1 ; PENG Haiping1 ; YANG Binfeng1 ; HUANG Bangrong1
1.Department of Oncology,Gansu Provincial Hospital of TCM,Gansu Lanzhou 730050,China;2.Department of Hematology,Zhangqiu District People's Hospital,Shandong Jinan 250200,China.
Keywords:
lung cancer; cinobufotalin; miR-497-5p; proliferation; apoptosis; angiogenesis
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.04.007
Abstract:
Objective:To investigate the effect of miR-497-5p/VEGFA pathway on proliferation,apoptosis and angiogenesis of lung cancer cells mediated by cinobufotalin.Methods:Lung cancer cell line A549 and H460 were selected for the study,and the cell lines were treated with different concentrations of cinobufotalin (0,25,50,75,100,125,150 μg/mL),and the cell viability was detected by MTT.Subsequently,groups were set up as control group,cinobufotalin group (100 μg/mL cinobufotalin),cinobufotalin+inhibitor NC group,and cinobufotalin+miR-497-5p inhibitor group,and cell viability was detected by MTT.Clone formation assay was performed to detect clone formation ability.Apoptosis was detected by flow cytometry.The protein expression of VEGF,VEGFA was detected by Western blot.miR-497-5p expression was detected by RT-qPCR.And miR-497-5p targeting to VEGFA was determined using luciferase reporter assay.Results:The proliferative viability of A549,H460 cells decreased significantly with the increase of cinobufotalin concentration (P＜0.01).Compared with the control group,the clone formation ability of A549,H460 cells was significantly reduced,apoptosis was significantly increased,VEGF and VEGFA protein expression was significantly reduced,and miR-497-5p mRNA expression was significantly increased in the cinobufotalin group (P＜0.01).Luciferase reporter assay showed that miR-497-5p targeted VEGFA.Compared with the cinobufotalin+inhibitor NC group,miR-497-5p mRNA expression was significantly lower,VEGF and VEGFA protein expression was significantly higher,cell clone formation ability was significantly increased,apoptosis was significantly inhibited,and cell proliferation viability was significantly increased in the A549,H460 cells of cinobufotalin+miR-497-5p inhibitor group (P＜0.05).Conclusion:Cinobufotalin may inhibit proliferation and angiogenesis of lung cancer cells,and induce cells apoptosis by regulating miR-497-5p/VEGFA pathway.

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