|Table of Contents|

Salidroside suppresses proliferation and migration of non-small cell lung cancer cells via miRNA-210-3p/E2F3

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 04
Page:
625-631
Research Field:
Publishing date:

Info

Title:
Salidroside suppresses proliferation and migration of non-small cell lung cancer cells via miRNA-210-3p/E2F3
Author(s):
YANG Qi1; 2 ; MING Yujun1; 2 ; WANG Xiujuan1; 2 ; JIANG Li2 ; CHEN Shaoping2
1.North Sichuan Medical College,Sichuan Nanchong 637000,China;2.Affiliated Hospital of North Sichuan Medical College,Sichuan Nanchong 637000,China.
Keywords:
salidroside; lung cancer; miRNA-210-3p; E2F3; proliferation; migration
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.04.006
Abstract:
Objective:To investigate the effect and mechanism of salidroside (Sal) on proliferation and migration of non-small cell lung cancer (NSCLC) cells through miRNA-210-3p/E2F3.Methods:The expression and survival effects of miR-210-3p and target gene in lung adenocarcinoma tissues and normal tissues were analyzed by bioinformatics sites.Immunohistochemistry was used to detect the expression of E2F3 protein in lung adenocarcinoma tissues and normal tissues.qRT-PCR was used to detect the expression of miR-210-3p and E2F3 in NSCLC cells.Western blot was used to detect the expression of E2F3 protein in NSCLC cells.The effects of Sal and miR-210-3p on proliferation,migration and colony formation of NSCLC cells were determined by CCK-8 assay,scratch assay and colony formation assay,respectively.Results:Bioinformatics site showed that miR-210-3p and E2F3 were highly expressed in lung adenocarcinoma tissues and were associated with poor prognosis.Sal inhibited the proliferation activity of NSCLC cells in a time-concentration dependent manner (P＜0.05),and significantly decreased the migration and colony formation ability of NSCLC cells (P＜0.05),while low concentrations of Sal had no significant inhibitory effect on the proliferation of normal lung epithelial cells.miRNA-210-3p expression and E2F3 expression were upregulated in NSCLC cells (P＜0.05),but Sal could inhibit their expression (P＜0.05).Compared with the control group,the expression of E2F3 was increased in the miR-210-3p inhibitor group (P＜0.05),inhibited in the miR-210-3p mimics group (P＜0.05).The combination of Sal and miR-210-3p mimics further inhibited the downregulation of E2F3 expression induced by miR-210-3p mimics and the enhancement of NSCLC cell proliferation and migration (P＜0.05).Conclusion:miR-210-3p and E2F3 were highly expressed in lung adenocarcinoma,and Sal could affect the biological behavior of NSCLC cells and exert anticancer effects via negative regulation of miRNA-210-3p/E2F3 in a time-concentration dependent manner.

References:

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Memo

Memo:
四川省医学会（恒瑞）科研基金专项科研课题（编号：2021HR24）；四川省科技计划项目（编号：2020YFS0572）；四川省南充市2022年市校科技战略合作专项（编号：22SXQT0152）
Last Update: 1900-01-01