|Table of Contents|

Berbamine induces the apoptosis of gastric cancer AGS cells via endoplasmic reticulum stress pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 04
Page:
595-599
Research Field:
Publishing date:

Info

Title:
Berbamine induces the apoptosis of gastric cancer AGS cells via endoplasmic reticulum stress pathway
Author(s):
XU HuaJIN YingLU XiaochunSONG YiQIAO Zhenguo
Department of Gastroenterology,Suzhou Ninth People's Hospital,Jiangsu Suzhou 215200,China.
Keywords:
gastric cancerberbamineapoptosischopendoplasmic reticulum stress
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2022.04.007
Abstract:
Objective:To investigate the effects and the possible mechanisms of berbamine on the cell proliferation and apoptosis of human gastric cancer AGS cells.Methods:Cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Cell apoptosis was detected by Annexin V/PI staining assay.The expression of Bcl-2,Bax,chop,GRP78 and cleaved caspase 12 proteins was detected by Western blot assay.Results:MTT assay result showed that berbamine could obviously inhibit the cell growth of AGS cells in a dosage-dependent manner.The cell apoptosis assay result showed that berbamine significantly increased the apoptotic rate of AGS cells.The Western blot result showed that berbamine down-regulated the expression of Bcl-2,while up-regulated the expression of Bax,chop,GRP78 and cleaved caspase 12 proteins.Moreover,compared with the berbamine group,the expression of chop in berbamine+chop siRNA group significantly decreased (P<0.01),while the expression of Bcl-2 in berbamine+chop siRNA group significantly increased (P<0.01).Conclusion:Berbamine could induce cell apoptosis via endoplasmic reticulum stress pathway in gastric cancer AGS cells.

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Memo

Memo:
苏州市第九人民医院科研项目(编号:201818)
Last Update: 1900-01-01