|Table of Contents|

lncRNA HOTAIR activates RhoA/ROCK pathway by targeting miR-126 and promotes the proliferation,invasion and EMT of Hep-2 cells in laryngeal cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 03
Page:
365-370
Research Field:
Publishing date:

Info

Title:
lncRNA HOTAIR activates RhoA/ROCK pathway by targeting miR-126 and promotes the proliferation,invasion and EMT of Hep-2 cells in laryngeal cancer
Author(s):
LI DenghuiCAI RunzhuoZHU LimingCHEN Dong
Department of Otolaryngology,Jinzhou Central Hospital,Liaoning Jinzhou 121000,China.
Keywords:
lncRNA HOTAIRmiR-126RhoA/ROCK pathwayHep-2 cellsproliferationinvasionEMT
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2022.03.001
Abstract:
Objective:To investigate the effects and mechanism of lncRNA HOTAIR on the proliferation,invasion and EMT of Hep-2 cells in laryngeal carcinoma.Methods:The expressions of lncRNA HOTAIR and miR-126 in laryngeal cancer tissues,adjacent tissues and Hep-2 cells were detected by RT-qPCR.After downregulating the expression of lncRNA HOTAIR,the proliferation,invasion and EMT of Hep-2 cells were detected by CCK-8,Transwell and Western blot,respectively.The target genes of HOTAIR were analyzed by miRcode,and the relationship between lncRNA HOTAIR and miR-126 was analyzed by double luciferase reporter gene assay.The expressions of lncRNA HOTAIR and miR-126 were up-regulated,and the effects of lncRNA HOTAIR through miR-126 on the proliferation,invasion and EMT of Hep-2 cells were detected.After the expression of miR-126 was down-regulated,the expressions of RhoA and ROCK proteins were detected by Western blot.The effects of down-regulation of miR-126 on the proliferation,invasion and EMT of Hep-2 cells were detected in Y-27632 treated-cells.Results:The expression of lncRNA HOTAIR was significantly up-regulated in Hep-2 cells and laryngeal carcinoma tissues(P<0.01),the miR-126 expression was significantly down-regulated in Hep-2 cells and laryngeal carcinoma tissues(P<0.01).Down-regulation of lncRNA HOTAIR inhibited the proliferation,invasion and EMT of Hep-2 cells.lncRNA HOTAIR and miR-126 had a targeted and negative regulatory relationship.RhoA/ROCK pathway in Hep-2 cells was activated after down-regulation of miR-126 expression,and the proliferation,invasion and EMT of Hep-2 cells were promoted.Conclusion:Up-regulation of lncRNA HOTAIR actives RhoA/ROCK pathway by targeting miR-126 to promote proliferation,invasion and EMT of Hep-2 cells in laryngeal cancer.

References:

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Memo

Memo:
辽宁省自然科学基金(编号:20170540559)
Last Update: 2021-12-31