|Table of Contents|

miR-124 inhibits the proliferation of glioma cells through targeted regulation of AURKA

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 06
Page:
930-934
Research Field:
Publishing date:

Info

Title:
miR-124 inhibits the proliferation of glioma cells through targeted regulation of AURKA
Author(s):
Shaya Mahati; Dina Ainiwaer; XIAO Shuangdong; LIU Wen; WANG Zengliang
First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China.
Keywords:
GBM; miR-124; proliferation; AURKA
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2021.06.005
Abstract:
Objective:To explore the effects of miR-124 and AURKA on the proliferation of glioma cells and explore its mechanism.Methods:The expression levels of miR-124 and AURKA in glioma tissues and non-tumor tissues were determined by PCR.Liposome miR-124 mimic carrying miR-124 and empty vector miR-124 were transfected into glioma cell line LN-229 and T98G,the effect of miR-124 on the proliferation of glioma cell lines LN-229 and T98G was observed by absorbance.The target gene of miR-124 was predicted by Targetscan software,and the binding site of miRNA-124 and AURKA was detected by Luciferase report.Real-time quantitative PCR method detects the changes of AURKA gene after miRNA-124 transfection.Results:Compared with non-glioma tissues,the expression of miR-124 was decreased in glioma tissues.MTT experiments showed that the proliferation ability of glioma cells in the high expression group of miR-124 was weakened.The proliferation ability of glioma cells in the miR-124 low expression group increased,the difference is statistically significant (P<0.05).Targetscan software suggested that AURKA may be the target gene of miR-124.Western blot and Luciferase experiments showed that compared with the negative control group,the expression of AURKA in the high expression group of miR-124 decreased,while the expression of AURKA in the low expression group of miR-124 increased.Conclusion:miR-124 is down-regulated in glioma cells and tissues,and may affect the proliferation and invasion of glioma cells by regulating AURKA.

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Memo

Memo:
National Natural Science Foundation of China(No.81801232）；国家自然科学基金青年科学基金项目（编号：81801232）
Last Update: 1900-01-01