|Table of Contents|

Expression of Keap1 and relationship with Ki67 index in colorectal adenocarcinoma tissues

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
458-462
Research Field:
Publishing date:

Info

Title:
Expression of Keap1 and relationship with Ki67 index in colorectal adenocarcinoma tissues
Author(s):
SUN Dejun1KONG Yonghe2
1.Surgery Staff Room,Department of Clinical Medicine,Qinghai Institute of Heath Sciences,Qinghai Xining 810000,China;2.Department of Radiotherapy,the Fifth People's Hospital of Qinghai Province(Cancer Hospital),Qinghai Xining 810000,China.
Keywords:
colorectal adenocarcinomaKeap1Ki67prognosis
PACS:
R735.35
DOI:
10.3969/j.issn.1672-4992.2021.03.021
Abstract:
Objective:To detect expression of Keap1 in colorectal adenocarcinoma tissues,analyze its relationship with Ki67 index.Methods:93 cases of colorectal adenocarcinoma were selected as observation group,and normal colorectal mucosa tissues(>3 cm from tumor edge) were selected as control group.Expression of Keap1 was detected by IHC and fluorescence PCR in two groups.Relationship between Keap1 and Ki67 proliferation index was analyzed in observation group.Results:IHC results showed that expression of Keap1 was significantly different in two groups.Positive rate of Keap1 was related to tumor maximum diameters,lymph node metastasis,vascular tumor thrombus,infiltration depth and TNM staging of observation groups.Positive rate of Keap1 was not related to gender,age,differentiation degree and cancer nodules.The expression of Keap1 was related to prognosis in observation group.Expression of Keap1 had a negative correlation with Ki67 proliferation index in observation group.Expression of Keap1 mRNA was statistically significant in two groups.Conclusion:The low expression of Keap1 may be associated with larger tumor diameter,more lymph node metastasis,presence of vascular cancer thrombus,deeper tumor infiltration and later TNM staging.Keap1 may play a role in inhibiting cell proliferation.Expression of Keap1 has certain value in judging prognosis in colorectal adenocarcinoma.

References:

[1]MATTHEW EM,YANG Z,PERI S,et al.Plk2 loss commonly occurs in colorectal carcinomas but not adenomas:Relationship to mTOR signaling[J].Neoplasia,2018,20(3):244-255.
[2]YANG CK,GUAN S,YING MG.Effects of CO2 pneumoperitoneum on the expression of thymidine kinase 1 and Ki67 in colorectal carcinoma cells[J].Sur Endoscopy,2014,28(10):2863-2870.
[3]JEONG Y,HOANG NT,LOVEJOY A,et al.Role of KEAP1/NRF2 and TP53 mutations in lung squamous cell carcinoma development and radiotherapy response prediction[J].Cancer Discovery,2017,7(1):86-101.
[4]MARGARITESCU C,PIRICI D,CHERCIU I,et al.CD133/CD166/Ki-67 triple immunofluorescence assessment for putative cancer stem cells in colon carcinoma[J].J Gastrointestin Liver Dis,2014,23(2):161-170.
[5]TANG J,GUI C,QIU S,et al.The clinicopathological significance of Ki67 in papillary thyroid carcinoma:A suitable indicator[J].World J Sur Oncol,2018,16(1):100-102.
[6]TOMONO A,ITOH T,YANAGITA E,et al.Cell cycle kinetic analysis of colorectal neoplasms using a new automated immunohistochemistry-based cell cycle detection method[J].Medicine,2015,94(4):501-503.
[7]DEL GOBBO A,PELLEGRINELLI A,GAUDIOSO G,et al.Analysis of NSCLC tumour heterogeneity,proliferative and 18F-FDG PET indices reveals Ki67 prognostic role in adenocarcinomas[J].Histopathology,2016,68(5):746-751.
[8]FABRIZIO FP,COSTANTINI M,COPETTI M,et al.Keap1/Nrf2 pathway in kidney cancer:Frequent methylation of KEAP1 gene promoter in clear renal cell carcinoma[J].Oncotarget,2017,8(7):11187-11198.
[9]AKDEMIR B,NAKAJIMA Y,INAZAWA J.miR-432 induces NRF2 stabilization by directly targeting KEAP1[J].Mol Cancer Res Mcr,2017,15(11):1570-1572.
[10]KLAPPROTH E,DICKREUTER E,ZAKRZEWSKI F,et al.Whole exome sequencing identifies mTOR and KEAP1 as potential targets for radiosensitization of HNSCC cells refractory to EGFR and β1 integrin inhibition[J].Oncotarget,2018,9(26):18099-18114.
[11]SKOWRON MA,NIEGISCH G,ALBRECHT P,et al.Various mechanisms involve the nuclear factor(erythroid-derived 2)-like(NRF2) to achieve cytoprotection in long-term cisplatin-treated urothelial carcinoma cell lines[J].Int J Mol Sci,2017,18(8):1680-1682.
[12]HAYASHI M,GUIDA E,GOLDBERG R,et al.GULP1 is an epigenetically altered and functional tumor suppressor in urothelial carcinoma through regulation of Nrf2-Keap1 signaling axis[J].Cancer Res,2017,77(13):1551-1552.
[13]HONG F,FREEMAN ML,LIEBLER DC.Identification of sensor cysteines in human keap1 modified by the cancer chemopreventive agent sulforaphane[J].Chemical Res Toxicology,2005,18(12):1917-1926.
[14]HIDEFUMI SASAKI,AYUMI SUZUKI,MASAYUKI SHITARA,et al.Keap1 mutations in lung cancer patients[J].Oncol Letters,2013,6(3):719-721.
[15]WEI J,ZHANG Y,LUO Y,et al.Aldose reductase regulates miR-200a-3p/141-3p to coordinate Keap1-Nrf2,Tgfβ1/2,and Zeb1/2 signaling in renal mesangial cells and the renal cortex of diabetic mice[J].Free Radical Biol Med,2014,67(7):91-102.
[16]HINTSALA HR,HAAPASAARI KM,SOINI Y,et al.An immunohistochemical study of NFE2L2,KEAP1 and 8-hydroxy-2'-deoxyguanosine and the EMT markers SNAI2,ZEB1 and TWIST1 in metastatic melanoma[J].Histol Histopathol,2016,32(2):11778-11779.

Memo

Memo:
-
Last Update: 2020-12-31