|Table of Contents|

Effect of PIM1 gene on proliferation,apoptosis,invasion and migration of esophageal cancer cells through regulating STAT3 signaling pathway protein expression

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
392-396
Research Field:
Publishing date:

Info

Title:
Effect of PIM1 gene on proliferation,apoptosis,invasion and migration of esophageal cancer cells through regulating STAT3 signaling pathway protein expression
Author(s):
XU Hanqiao; PAN Jie; TIAN Xuetao; CHEN Zhiqiang
Department of Thoracic Surgery,Wuhan No.6 Hospital,Jianghan University Affiliated Hospital,Hubei Wuhan 430015,China.
Keywords:
PIM1 gene; STAT3 signaling pathway; proliferation; apoptosis; invasion; migration
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2021.03.006
Abstract:
Objective：To investigate the effect of proto-oncogene PIM1 gene on proliferation,apoptosis,invasion and migration of esophageal cancer KYSE150 cells by regulating signal transducer and activator 3 (STAT3) signaling pathway.Methods:KYSE150 cells were transfected with PIM1 siRNA and siRNA Control,respectively named PIM1-siRNA group and NC group,and a blank control group was set.Real-time quantitative PCR (qRT-PCR) and Western blot were used to detect the expression of PIM1 in each group.Cell proliferation,apoptosis,invasion and migration were measured by MTT assay,flow cytometry and Transwell assay,respectively.Western blot was used to detect protein expression levels related to cell proliferation,apoptosis,invasion and migration,and STAT3 signaling pathway.Results:The expression of PIM1 mRNA and protein in KYSE150 cells of PIM1-siRNA group was significantly lower than that of Control group (P＜0.05).The OD value,invasion and migration number of KYSE150 cells in PIM1-siRNA group were significantly lower than those in Control group (P＜0.05),and the apoptosis rate was significantly higher than that in Control group (P＜0.05).PCNA,MMP-2,MMP-9 and the expression level of p-STAT3 protein was lower than that of Control group (P＜0.05),and the expression level of Cleaved Caspase-3 protein was higher than that of Control group.There was no significant difference between the Control group and the NC group (P＞0.05).Conclusion:PIM1 regulates proliferation,apoptosis,invasion and migration of esophageal cancer KYSE150 cells by regulating STAT3 signaling pathway.

References:

［1］LEE K,KIM HR,PARK SI,et al.Surgical outcome of colon interposition in esophageal cancer surgery:Analysis of risk factors for conduit-related morbidity［J］.Thorac Cardiovasc Surg,2017,66(05):384-389.
［2］HASSANIPOUR S,FATHALIPOUR M,SALEHINIYA H.The incidence of prostate cancer in Iran:A systematic review and meta-analysis［J］.Health Promotion Perspectives,2017,6(2):41-45.
［3］THUMALLAPALLY N,MESHREF A,MOUSA M,et al.Survival benefit of neoadjuvant versus adjuvant radiotherapy in lymph node positive esophageal cancer:a population based analysis［J］.J Gastrointest Oncol,2017,8(5):825-832.
［4］NARLIK-GRASSOW M,BLANCO-APARICIO C,CARNERO A.The PIM family of serine/threonine kinases in cancer［J］.Med Res Rev,2013,34(1):136-159.
［5］LI JQ,YANG X,ZHOU XM.PIM1 gene silencing inhibits proliferation and promotes apoptosis of human esophageal cancer cell line Eca-109［J］.Cancer Biomarker,2017,18(2):149-154.
［6］MOLOGNI L,MAGISTRONI V,CASUSCELLI F,et al.The novel PIM1 inhibitor NMS-P645 reverses PIM1-dependent effects on TMPRSS2/ERG positive prostate cancer cells and shows anti-proliferative activity in combination with PI3K inhibition［J］.J Cancer,2017,8(1):140-145.
［7］ZHANG M,LIU T,SUN H,et al.Pim1 supports human colorectal cancer growth during glucose deprivation by enhancing the Warburg effect［J］.Cancer Sci,2018,109(5):1468-1479.
［8］MAHONY R,GARGAN S,ROBERTS KL,et al.A novel anti-viral role for STAT3 in IFN-α signalling responses［J］.Cell Mol Life Sci,2017,74(9):1-10.
［9］YANG JG,LU R,YE XJ,et al.Icaritin reduces oral squamous cell carcinoma progression via the inhibition of STAT3 signaling［J］.Int J Mol Sci,2017,18(1):132-141.
［10］PAULIN R,COURBOULIN A,MELOCHE J,et al.Signal transducers and activators of transcription-3/pim1 axis plays a critical role in the pathogenesis of human pulmonary arterial hypertension［J］.Circulation,2011,123(11):1205-1215.
［11］SHIMADA H.p53 molecular approach to diagnosis and treatment of esophageal squamous cell carcinoma［J］.Ann Gastroenterol Surg,2018,2(4):266-273.
［12］DI PM,CANTO MI,FITZGERALD RC.Endoscopic management of early adenocarcinoma and squamous cell carcinoma of the esophagus-screening,diagnosis,and therapy［J］.Gastroenterology,2017,154(2):421-436.
［13］YANG J,LIU K,YANG J,et al.PIM1 induces cellular senescence through phosphorylation of UHRF1 at Ser311［J］.Oncogene,2017,36(34):4828-4842.
［14］BOGUSZ J,ZRUBEK K,REMBACZ KP,et al.Structural analysis of PIM1 kinase complexes with ATP-competitive inhibitors［J］.Sci Rep,2017,7(1):13399-13410.
［15］MAWAS AS,AMATYA VJ,SUZUKI R,et al.PIM1 knockdown inhibits cell proliferation and invasion of mesothelioma cells［J］.Int J Oncol,2017,50(3):1029-1034.
［16］ZHAO W,QIU R,LI P,et al.PIM1:A promising target in patients with triple-negative breast cancer［J］.Med Oncol,2017,34(8):142-147.
［17］IYER RS,CHATHAM L,SLEIGH R,et al.A functional SUMO-motif in the active site of PIM1 promotes its degradation via RNF4,and stimulates protein kinase activity［J］.Sci Rep,2017,7(1):3598-3610.
［18］YE C,ZHANG C,HUANG H,et al.The natural compound myricetin effectively represses the malignant progression of prostate cancer by inhibiting PIM1 and disrupting the PIM1/CXCR4 interaction［J］.Cell Physiol Biochem,2018,48(3):1230-1244.
［19］GUO S,FAN J,WANG B,et al.Highly selective red-emitting fluorescent probe for imaging cancer cells in situ by targeting Pim-1 kinase［J］.Acs Appl Mater Interfaces,2018,10(2):1499-1507.
［20］陈建丽,陈晓文.Pim1蛋白在食管不同病变组织中的表达及临床意义［J］.新乡医学院学报,2018,35(3):201-203. CHEN JL,CHEN XW.Expression and clinical significance of Pim1 protein in different esophageal tissues［J］.Journal of Xinxiang Medical College,2018,35(3):201-203.
［21］HAN Y,YANG X,ZHAO N,et al.Alpinumisoflavone induces apoptosis in esophageal squamous cell carcinoma by modulating miR-370/PIM1 signaling［J］.Am J Cancer Res,2016,6(12):2755-2771.
［22］CHOE KN,MOLDOVAN GL.Forging ahead through darkness:PCNA,still the principal conductor at the replication fork［J］.Mol Cell,2017,65(3):380-392.
［23］WANG Y,GAO W,SHI X,et al.Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a gasdermin［J］.Nature,2017,547(7661):99-103.
［24］PIROMKRAIPAK P,SANGPAIROJ K,TIRAKOTAI W,et al.Cysteinyl leukotriene receptor antagonists inhibit migration,invasion,and expression of MMP-2/9 in human glioblastoma［J］.Cell Mol Neurobiol,2018,38(2):559-573.
［25］ZHOU C,MA J,SU M,et al.Down-regulation of STAT3 induces the apoptosis and G1 cell cycle arrest in esophageal carcinoma ECA109 cells［J］.Cancer Cell Int,2018,18(1):53-64.

Memo

Memo:
-
Last Update: 2020-12-31