|Table of Contents|

Effect of inhibiting TPD52 on apoptosis and ROS content in colorectal cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 04
Page:
569-573
Research Field:
Publishing date:

Info

Title:
Effect of inhibiting TPD52 on apoptosis and ROS content in colorectal cancer cells
Author(s):
Huang Jianwei1; Li Chaohui2; Han Baowei2
1.Pathology Department;2.Gastrointestinal Surgery Department,Luoyang Central Hospital,Henan Luoyang 471000,China.
Keywords:
colorectal cancer; TPD52 gene; apoptosis; ROS content
PACS:
R735.3+5;R735.3+7
DOI:
10.3969/j.issn.1672-4992.2019.04.007
Abstract:
Objective:To investigate the effect of inhibition of TPD52 gene expression on cell viability,apoptosis rate and ROS content in colorectal cancer cells.Methods:Normal colon NCM460 cells were used as control cells,and RT-PCR and Western blotting were used to detect the mRNA and protein expression of TPD52 in Caco2,SW480,HCT116,HT29 cells in colorectal cancer.LipofectamineTM 2000 was used as a vector.siRNA inhibition of TPD52 expression(TPD52-siRNA) and negative control siRNA (NC group) were transfected into HCT116 cells.MTT was used to detect cell viability cells transfected for 48 h,and apoptosis rate and ROS content were detected by flow cytometry.Protein expression of Ki67,PCNA,Bax and Survivin were detected by Western blotting.Results:Compared with normal colon NCM460 cells,the mRNA and protein expression of TPD52 in Caco2,SW480,HCT116 and HT29 cells increased significantly,and the difference was statistically significant (P＜0.05).The mRNA and protein expression of TPD52 in HCT116 cells transfected with TPD52-siRNA were significantly decreased,and the difference was statistically significant compared with that of the blank control group and the NC group (P＜0.05).Compared with the NC group,the cell vitality decreased obviously in TPD52-siRNA group,the apoptosis rate increased,the ROS content increased,the protein expression of Ki67,PCNA and Survivin decreased obviously,and the protein expression of Bax increased obviously,the difference was statistically significant (P＜0.05).Conclusion:The inhibition of TPD52 gene expression can reduce the activity of colorectal cancer cells and induce apoptosis.The mechanism may be related to the increase of intracellular ROS level and down regulation of Ki67,PCNA and Survivin expression and up regulation of Bax expression.

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