|Table of Contents|

Sodium arsenite combined with tetrandrine inhibits the proliferation of MDA-MB-231 cells in breast cancer and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 04
Page:
546-551
Research Field:
Publishing date:

Info

Title:
Sodium arsenite combined with tetrandrine inhibits the proliferation of MDA-MB-231 cells in breast cancer and its mechanism
Author(s):
Yu Bowen1; 2; Fan Yingyi2; Guo Yubo1; 2; Pei Xiaohua3
1.Beijing University of Chinese Medicine,Beijing 100029,China;2.Galactophore Department,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;3.Fangshan Tradition Medical Hospital of Beijing,Beijing 102488,China.
Keywords:
breast cancer; sodium arsenite; tetrandrine; proliferation inhibition; apoptosis
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2019.04.002
Abstract:
Objective:To investigate the effects of sodium arsenite combined with tetrandrine on proliferation inhibition in human breast cancer cell line MDA-MB-231.Methods:Cell proliferation was measured using MTT assay.Cell apoptosis was examined by Annexin V-FITC/PI double staining analysis.Chromatin condensation was observed by Hoechst33258 staining.Cell cycle was examined by PI staining.The expression of p-GSK3β,GSK3β and apoptosis-related proteins PARP,bcl-2 were detected by Western blot.Results:Both alone sodium arsenite and alone tetrandrine could inhibit the proliferation of MDA-MB-231 cells (P＜0.01).Compared with each drug alone,the combination could enhance the inhibition rate (P＜0.000 1).Apoptosis was induced significantly by the combination.Chromatin condensation was observed,and the apoptosis rate was dose-dependent.After treatment with NaAsO2 10 μmol/L+tetrandrine 4 μg/ml for 48 h,the rate of S phase was increased significantly (P＜0.05).After treatment with NaAsO2 15 μmol/L+tetrandrine 4.5 μg/ml for 48 h,the rate of G2/M phase was increased significantly (P＜0.001).After treatment with the combination,the expression levels of PARP,p-GSK3β,GSK3β were up-regulated (P＜0.000 1),while bcl-2 was down-regulated (P＜0.05).Conclusion:Sodium arsenite combined with tetrandrine could inhibit the proliferation of MDA-MB-231 cells.The mechanism was related to cell cycle S phase or G2/M phase arrest,the up-regulation of GSK3β protein,and apoptosis induction.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.81560775）；国家自然科学基金(编号：81560775);北京市自然科学基金(编号：7182098)
Last Update: 1900-01-01