|Table of Contents|

Effect of microRNA-653 on biological characteristics of human non-small cell lung cancer cells by targeting OIP5 gene and regulating mTOR signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 06
Page:
831-837
Research Field:
Publishing date:

Info

Title:
Effect of microRNA-653 on biological characteristics of human non-small cell lung cancer cells by targeting OIP5 gene and regulating mTOR signaling pathway
Author(s):
Liang Yuan1; Feng Yangyang2; Li Linlin1; Shen Xiaoyu1; Xin Tian1; Zhao Yuwei1; Ma Rui1
1.Department of No.4 Internal Medicine,Liaoning Cancer Hospital,Liaoning Shenyang 110042,China;2.Institute of Pathology and Pathophysiology,College of Basic Medical Science,China Medical University,Liaoning Shenyang 110122,China.
Keywords:
microRNA-653; OIP5; mTOR signaling pathway; non-small cell lung cancer
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2018.06.005
Abstract:
Objective:To explore the impact of microRNA-653(miR-653) on proliferation and apoptosis of non-small cell lung cancer(NSCLC) cells by targeting OIP5 gene and regulating mTOR signaling pathway.Methods:Human normal endothelial cell line(BEAS-2B) and four NSCLC cell lines(H1650,H1975,A549 and H292) were assigned into the control,mimics,mimics-negative control(NC),inhibitor and inhibitor-NC groups.Quantitative RT-PCR and Western blot were applied to detect the expressions of miR-653,OIP5 and mTOR.MTT,scratch test,Transwell and flow cytometry were carried out to analyze the effect of miR-653 on cell proliferation,migration,invasion,apoptosis and cycle distribution and regulation of mTOR signaling pathway.Results:Compared with the control and mimics groups,cell proliferation,migration and invasion abilities decreased in the mimics group.Additionally,cell apoptosis rate increased and cells were mostly in G1 phase.miR-653 inhibited the activation of mTOR signaling pathway.Compared with the control and inhibitor-NC group,mTOR signaling pathway was activated and cell proliferation,migration and invasion abilities increased in the inhibitor group,and cell apoptosis rate decreased and cells were less in G1 phase.Conclusion:These results suggest that miR-653 expression was low in NSCLC cells and influenced NSCLC cells by inhibiting OIP5 gene expression and negatively regulating mTOR signaling pathway.

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Memo

Memo:
辽宁省自然科学基金项目（编号：20170540562）
Last Update: 2018-01-29