|Table of Contents|

CUL4A promote human gastric cancer cell proliferation,invasion and metastasis and its mechanisms

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 04
Page:
502-505
Research Field:
Publishing date:

Info

Title:
CUL4A promote human gastric cancer cell proliferation,invasion and metastasis and its mechanisms
Author(s):
Li Yan; Tian Xin; Meng Fandong; Sui Chengguang; Wu Di; Fu Liye; Jiang Youhong
Cancer Research Institute,the First Affiliated Hospital,China Medical University,Liaoning Shenyang 110001,China.
Keywords:
CUL4A; gastric cancer; SCG-7901 cell; Snail; E-cadherin; ZEB1
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2018.04.004
Abstract:
Objective:To investigate the mechanism of CUL4A gene on the proliferation and invasion in gastric cancer.Methods:siRNA interference the expression of CUL4A in gastric cancer SCG-7901 cells.Using CCK-8 method to detect the proliferation of each group cells.To detect the invasion of each group cells by Transwell chambers.To detect each group cells apoptosis by Annexin V-FITC/PI method,and analysis the effects of CUL4A on cell apoptosis.Using Real time-PCR to analyze the expression of EMT related genes in gastric cancer cells.Results:siRNA interferenced the expression of CUL4A gene effectively in SCG-7901 gastric cancer cells（P＜0.05）.siRNA CUL4A inhibited the proliferation of gastric cancer cell（P＜0.05）.The results of Transwell chambers,interference CUL4A hindered the invasion of SCG-7901 cells（P＜0.05）.According to the results of cell apoptosis,siRNA CUL4A gene promoted the apoptosis of cells（P＜0.05）.Real time-PCR results showed that the expression of Snail mRNA and ZEB1 mRNA were lower than other group cells（P＜0.05）,the expression of E-cadherin mRNA was increased（P＜0.05）.Conclusion:CUL4A gene promoted gastric cancer cell proliferation,inhibited apoptosis and inhibition,and affected the expression of EMT related genes.

References:

[1]Zhao ZS,Wang YY,Chu YQ,et al.SPARC is associated with gastric cancer progression and poor survival of patients［J］.Clin Cancer Res,2010,16:260-268.
[2]Y Sang,F Yan,X Ren.The role and mechanism of CRL4E3 ubiquit in ligase in cancer and its potential therapy implications［J］.Oncotarget,2015,6(40):42590-42602.
[3]L Liu,S Lee,J Zhang,et al.CUL4A abrogation augments DNA damage response and protection against skin carcinogenesis［J］.Molecular Cell,2009,34:451-460.
[4]J Hannah,PB Zhou.The CUL4A ubiquit in ligase is a potential therapeutic target in skin cancer and other malignancies［J］.Chinese J Cancer,2013,32:478-482.
[5]Jackson S,Xiong Y.CRL4S:The CUL4-RING E3 ubiquitin ligases［J］.Trends Biochem Sci,2009,34(11):562-570.
[6]U Bedi,VK Mishra,D Wasilewski,et al.Epigenetic plasticity:A central regulator of epithelial-to-mesenchymal transition in cancer［J］.Oncotarget,2014,5:2016-2029.
[7]A Puisieux,T Brabletz,J Caramel.Oncogenic roles of EMT-inducing transcription factors［J］.Nature Cell Biol,2014,16:488-494.
[8]WL Tam,RA Weinberg.The epigenetics of epithelial-mesenchymal plasticity in cancer［J］.Nature Medicine,2013,19:1438-1449.
[9]Song J，Zhang J，Shao J.Knockdown of CUL4A inhibits invasion and induces apoptosis in osteosarcoma cells［J］.Int J Immunopathol Pharmacol,2015,28(2):263-269.
[10]Y Wang,M Wen,Y Kwon,et al.CUL4A induces epithelial-mesenchymal transition and promotes cancer metastasis by regulating ZEB1 expression［J］.Cancer Res,2014,74:520-531.
[11]YL Yang,MS Hung,Y Wang,et al.Lung tumourigenesis in a conditional CUL4A transgenic mouse model［J］.J Pathol,2014,233:113-123.
[12]Gheldof A,Berx G.Cadherins and epithelial-to-mesenchymal transition［J］.Prog Mol Biol Transl Sci,2013,116:317-336.
[13]Chen D,Wang J,Zhang Y,et al.Effect of down-regulated transcriptional repressor ZEB1 on the epithelial-mesenchymal transition of ovarian cancer cells［J］.Int J Gynecol Cancer,2013,23(8):1357-1366.

Memo

Memo:
-
Last Update: 2017-12-29