|Table of Contents|

Synergistic radiosensitization of salinomycin on nasopharyngeal carcinoma CNE-2 cell

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 04
Page:
498-501
Research Field:
Publishing date:

Info

Title:
Synergistic radiosensitization of salinomycin on nasopharyngeal carcinoma CNE-2 cell
Author(s):
Wu Danxin1; Luo Zhiguo1; Ming Bangchun1; Wang Dan1; Wang Senming2
1.Department of Oncology,Taihe Hosipital,Hubei Unversity of Medicine,Hubei Shiyan 442000,China;2.Department of Oncology,Zhujiang Hosipital,Southern Medical Unversity,Guangdong Guangzhou 510282,China.
Keywords:
nasopharyngeal carcinoma; salinomycin; irradiation; radiosensitization
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2018.04.003
Abstract:
Objective:To investigate the effect of salinomycin on radiosensitization of nasopharyngeal carcinoma CNE-2 cell line．Methods:CCK8 method was used to detect the effect of combination treatment with salinomycin and irradiation on proliferation.Plate clone assay was used to measure the clone formation rate.Hoechst33258 staining was used to perform morphological assessment of apoptotic cells.Cell cycle distribution and apoptosis were analyzed by flow cytometry．Results:CCK8 assay showed exposure to combination of salinomycin and irradiation resulted in decline of CNE-2 cell viability rate in concentration dose or dose-dependent manner and combination treatment had an additive inhibitory effect.Plate clone assay showed the combination treatment resulted in reduction of clone formation.In Hoechst33258 staining assay,apoptotic bodies of NPC cells added in response to the combination treatment.FCM assay showed the combination treatment induced strongly augmented number of apoptotic cells induced apoptosis and blocked cell cycle G1／S phase.Conclusion:Our study shows that salinomycin may enhance radiosensitization on CNE-2 cells．The radiosensitivity enhancement of may be related to apoptosis and cell cycle arrest．

References:

[1]Yu MC,JM Yuan.Epidemiology of nasopharyngeal carcinoma［J］.Semin Cancer Biol,2002,12(6):421-429.
[2]Gupta PB,Onder TT,Jiang G,et al.Identification of selective inhibitors of cancer stem cells by high-throughput screening［J］.Cell,2009,138(4):645-659.
[3]Zhang Z,Zhao J,Mi Z,et al.Effects of salinomycin and 17AAG on proliferation of human gastric cancer cells in vitro［J］.Mol Med Rep,2017,16(2):1063-1070.
[4]Dosch JS,Ziemke EK,Shettigar A,et al.Cancer stem cell marker phenotypes are reversible and functionally homogeneous in a preclinical model of pancreatic cancer［J］.Cancer Res,2015,75(21):4582-4592.
[5]Tung CL,Chen JC,Wu CH,et al.Salinomycin acts through reducing AKT-dependent thymidylate synthase expression to enhance erlotinib-induced cytotoxicity in human lung cancer cells［J］.Exp Cell Res,2017,357(1):59-66.
[6]Liffers ST,Tilkorn DJ,Stricker I,et al.Salinomycin increases chemosensitivity to the effects of doxorubicin in soft tissue sarcomas［J］.BMC Cancer,2013,13:490.
[7]Kim JH,Yoo HI,Kang HS,et al.Salinomycin sensitizes antimitotic drugs-treated cancer cells by increasing apoptosis via the prevention of G2 arrest［J］.Biochem Biophys Res Commun,2012,418(1):98-103.
[8]Kim WK,Kim JH,Yoon K,et al.Salinomycin,a p-glycoprotein inhibitor,sensitizes radiation-treated cancer cells by increasing DNA damage and inducing G2 arrest［J］.Invest New Drugs,2012,30(4):1311-1318.
[9]Wang F,He L,Dai WQ,et al.Salinomycin inhibits proliferation and induces apoptosis of human hepatocellular carcinoma cells in vitro and in vivo［J］.PLoS One,2012,7(12):e50638
[10]Wang F,Dai W,Wang Y,et al.The synergistic in vitro and in vivo antitumor effect of combination therapy with salinomycin and 5-fluorouracil against hepatocellular carcinoma［J］.PLoS One,2014，9(5):e97414.
[11]Lieke T,Ramackers W,Bergmann S,et al.Impact of Salinomycin on human cholangiocarcinoma:Induction of apoptosis and impairment of tumor cell proliferation in vitro［J］.BMC Cancer,2012,12:466.
[12]Naujokat C,R Steinhart.Salinomycin as a drug for targeting human cancer stem cells［J］.J Biomed Biotechnol,2012,20(12):e950658.
[13]Wu DX,Wang SM,Zhang JR,et al.Effect of salinomycin on the proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cell line［J］.Journal of Practical Medicine,2013,29(24):3967-3970.［吴丹心，汪森明，张积仁,等.盐霉素诱导鼻咽癌CNE-2细胞凋亡及其作用机制的探讨［J］.实用医学杂志,2013,29(24):3967-3970.］
[14]Callaway TR,Edrington TS,Rychlik JL,et al.Ionophores:Their use as ruminant growth promotants and impact on food safety［J］.Curr Issues Intest Microbiol,2003,4(2):43-51.
[15]Fuchs D,Heinold A,Opelz G,et al.Salinomycin induces apoptosis and overcomes apoptosis resistance in human cancer cells［J］.Biochem Biophys Res Commun,2009,390(3):743-749.
[16]Daman Z,Montazeri H,Azizi M,et al.Polymeric micelles of PEG-PLA copolymer as a carrier for salinomycin against gemcitabine-resistant pancreatic cancer［J］.Pharm Res,2015,32(11):3756-3767.
[17]Zhou J,Li P,Xue X,et al.Salinomycin induces apoptosis in cisplatin-resistant colorectal cancer cells by accumulation of reactive oxygen species［J］.Toxicol Lett,2013,222(2):139-145.
[18]Ketola K,Hilvo M,Hyotylainen T,et al.Salinomycin inhibits prostate cancer growth and migration via induction of oxidative stress［J］.Br J Cancer,2012,106(1):99-106.
[19]Kusunoki Soshi,Kato Kiyoko,Tabu Kouichi,et al.The inhibitory effect of salinomycin on the proliferation,migration and invasion of human endometrial cancer stem-like cells［J］.Gynecologic Oncology,2013,129(3):598-605.
[20]Yu SM,SJ Kim.Salinomycin causes migration and invasion of human fibrosarcoma cells by inducing MMP-2 expression via PI3-kinase,ERK-1/2 and p38 kinase pathways［J］.Int J Oncol,2016,48(6):2686-2692.
[21]Hanrahan K,O'Neill A,Prencipe M,et al.The role of epithelial-mesenchymal transition drivers ZEB1 and ZEB2 in mediating docetaxel-resistant prostate cancer［J］.Mol Oncol,2017,11(3):251-265.
[22]Kuo SZ,Blair KJ,Rahimy E,et al.Salinomycin induces cell death and differentiation in head and neck squamous cell carcinoma stem cells despite activation of epithelial-mesenchymal transition and Akt［J］.BMC Cancer,2012,12:556.
[23]Pawlik TM,K Keyomarsi.Role of cell cycle in mediating sensitivity to radiotherapy［J］.Int J Radiat Oncol Biol Phys,2004,59(4):928-942.

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Memo:
十堰市太和医院院级基金资助项目（编号： 2015JJXM01）
Last Update: 2017-12-29