|Table of Contents|

Construction and identification of dual fluorescent protein reporter gene vector enhanced by four copies ARE

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 03
Page:
325-328
Research Field:
Publishing date:

Info

Title:
Construction and identification of dual fluorescent protein reporter gene vector enhanced by four copies ARE
Author(s):
Xu Hairong1; 2; Ren Xuedi2; Zhai Da2; Ma Yao2; Li Jing2; Dai Erxun1
1.Department of Oncology，Clinical College of Yangzhou University，Jiangsu Yangzhou 225001，China；2.Jiangsu Key Laboratory Integrated Traditional Chinese & Western，Jiangsu Yangzhou 225001，China.
Keywords:
ARE; dual fluorescent protein; reporter gene; vector construction
PACS:
R730.53
DOI:
10.3969/j.issn.1672-4992.2018.03.001
Abstract:
Objective：To construct the dual fluorescent protein reporter gene vector enhanced by four copies ARE.Methods：Three synthetic oligonucleotide ARE motifs were annealed and purified then inserted into the vector pARE-TK-GFP to construct the recombinant reporter vector p4ARE-TK-GFP.A red fluorescent protein and promoter fragment was cloned into the vector pMD18-T from pDsRed2-N1 to construct the vector pMD-DsRed.The vectors pMD-DsRed and p4ARE-TK-GFP were digested by Ade I and BspT I.The red fluorescent protein and promoter fragments were cloned into the vector p4ARE-TK-GFP to construct the dual fluorescent protein reporter gene vector.Results：The recombinant dual fluorescent protein reporter gene vector p4ARE-TK-GFP/DsRed was identified that it was successfully transformed into E.coli DH5α and expressed by restriction enzyme digestion and DNA sequencing.Conclusion：The dual fluorescent protein reporter gene vector enhanced by four copies ARE was successfully constructed and expressed in E.coli DH5α.This study lays a foundation for further research in the function of ARE.

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Memo

Memo:
国家自然科学基金资助项目（编号：81303109）；教育部博士点基金资助项目（编号：20113250120006）；扬州市自然科学基金资助项目（编号：YZ2014047）
Last Update: 1900-01-01