|Table of Contents|

Silencing of GRP94 inhibits the invasion and migration of cervical cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 02
Page:
186-190
Research Field:
Publishing date:

Info

Title:
Silencing of GRP94 inhibits the invasion and migration of cervical cancer cells
Author(s):
Xu LeiZhu XiaoyingDeng Xiaoyang
Department of Gynecology,the First Affiliated Hospital of Chengdu Medical College,Sichuan Chengdu 610500,China.
Keywords:
cervical cancerGRP94invasionmigrationCaSki cells
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2018.02.007
Abstract:
Objective:To investigate the expression of GRP94 in different cervical cancer cell lines and its effect on the invasion and migration of cells.Methods:Western blot and qRT-PCR were performed to detect the expression level of GRP94 in different cervical cancer cell lines.The cell line with high expression of GRP94 was selected.The expression of GRP94 in selected cervical cancer cell line was knocked down by using RNA interference technique.The change of cells' invasion and migration ability was detected by wound-healing assays and Transwell invasion assays.Results:The expression of GRP94 in all different cervical cancer cell lines(C33A,CaSki,HeLa,HeLa 229,MS751,ME-180,SiHa and HCC 94) was higher than normal cervical cells,and it was high in CaSki and MS751,but moderate in ME-180,SiHa and HCC 94,and low in C33A,HeLa and HeLa 229.The CaSki was selected to knock down the expression of GRP94,wound-healing assays and Transwell invasion assays showed that the invasion and migration ability of cells decreased significantly after down regulated GRP94.Conclusion:GRP94 is highly expressed in cervical cancer cells.Silencing of GRP94 can inhibit the invasion and migration ability of cervical cancer cells.

References:

[1] Akerman GS,Tolleson WH,Brown KL,et al.Human papillomavirus type16 E6 and E7 cooperate to increase epidermal growth factor receptor(EGFR) mRNA levels,overcoming mechanisms by which excessive EGFR signaling shortens the life span of normal human keratinocytes[J].Cancer Res,2001,61(9):3837-3843.
[2] Avruch J,Zhou D,Bardeesy N.YAP oncogene overexpression supercharges colon cancer proliferation[J].Cell Cycle,2012,11(6):1090-1096.
[3] Zhang W,Hou T,Niu C,et al.B3GNT3 expression is a novel marker correlated with pelvic lymph node metastasis and poor clinical outcome in early-stage cervical cancer[J].PLoS One,2015,10(12):e0144360.
[4] Yang L,Jia X,Li N,et al.Comprehensive clinic-pathological characteristics of cervical cancer in Southwestern China and the clinical significance of histological type and lymph node metastases in young patients[J].PLoS One,2013,8(10):e75849.
[5] Marzec M,Eletto D,Argon Y.GRP94:An HSP90-like protein specialized for protein folding and quality control in the endoplasmic reticulum[J].Biochimica et Biophysica Acta,2012,1823(3):774-787.
[6] Lee AS.Glucose regulated proteins in cancer:Molecular mechanisms and therapeutic potential[J].Nature Reviews Cancer,2014,14(4):263-276.
[7] Hong F,Liu B,Chiosis G,et al.α7 helix region of αI domain is crucial for integrin binding to endoplasmic reticulum chaperone gp96:A potential therapeutic target for cancer metastasis[J].Journal of Biological Chemistry,2013,288(25):18243-18248.
[8] Zhuang Yuyu,Yan Qin,Jiang Feizhou,et al.Expression and significance of GRP94 and CD8 in the pathological tissues of cervix[J].Progress in Modern Biomedicine,2011,17(11):3233-3239.[ 庄玉玉,严沁,姜飞洲,等.GRP94和CD8在宫颈病变组织中的表达及意义[J].现代生物医学进展,2011,17(11):3233-3239.]
[9] He C,Mao D,Hua G,et al.The Hippo/YAP pathway interacts with EGFR signaling and HPV oncoproteins to regulate cervical cancer progression[J].EMBO Molecular Medicine,2015,7(11):1426-1449.
[10] Tong YQ,Liu B,Zheng HY,et al.Overexpression of BMI-1 is associated with poor prognosis in cervical cancer[J].Asia Pac J Clin Oncol,2012,8(4):e55-62.
[11] Zhang W,Ou J,Lei F,et al.C14ORF166 overexpression is associated with pelvic lymph node metastasis and poor prognosis in uterine cervical cancer[J].Tumor Biol,2016,37(1):369-379.
[12] Zhang L,Huang H,Zhang L,et al.URG4 overexpression is correlated with cervical cancer progression and poor prognosis in patients with early-stage cervical cancer[J].BMC Cancer,2014,14:885.
[13] Lee AS.The glucose-regulated proteins:Stress induction and clinical applications[J].Trends Biochem Sci,2001,26(8):504-510.
[14] Langer R,Feith M,Siewert JR,et al.Expression and clinical significance of glucose regulated proteins GRP78(BiP) and GRP94(GP96) in human adenocarcinomas of the esophagus[J].BMC Cancer,2008,8:70.
[15] Wang Qiuhong,He Xiaohua,Zhang Jinye.Expression and significance of GRP94 in human hepatocellular carcinomas with HBV infection[J].Modern Oncology,2014,22(10):2364-2368.[王秋红,和小华,张金业.GRP94在HBV感染的人肝细胞肝癌中的表达及意义[J].现代肿瘤医学,2014,22(10):2364-2368.]
[16] Martínez-Aranda A,Hernández V,Guney E,et al.FN14 and GRP94 expression are prognostic/predictive biomarkers of brain metastasis outcome that open up new therapeutic strategies[J].Oncotarget,2015,6(42):44254-44273.

Memo

Memo:
四川省教育厅重点项目课题(编号:17ZA0128)
Last Update: 2017-11-30