|Table of Contents|

Preparation of ALK separate probes and detection of clinical by FISH

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 01
Page:
28-31
Research Field:
Publishing date:

Info

Title:
Preparation of ALK separate probes and detection of clinical by FISH
Author(s):
Cheng Hongxia1Ye Lun2
1.Wuhan Huaxia University of Technology,Hubei Wuhan 430223,China;2.Wuhan Health Care Biotechnology Co.,Ltd,Hubei Wuhan 430079,China.
Keywords:
ALK geneseparate probesFISHpreparationclinical detection
PACS:
R730.43
DOI:
10.3969/j.issn.1672-4992.2018.01.007
Abstract:
Objective:To establish separate probe for many kinds of ALK fusion gene detection by FISH to clinical samples.Methods:Constructed ALK separation probes of FISH.The detection effect of probes were evaluated by TFG-ALK and EML4-ALK samples and clinical tissue samples of NSCLC.Results:The ALK separate probe may detect TFG-ALK and EML4-ALK accurately.Conclusion:The ALK separate probe may detect gene arrangement among ALK and different genes.The probes have excellent fluorescent signal and low background interference.

References:

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Last Update: 2017-11-30