|Table of Contents|

Silencing of Y-box binding protein-1 suppresses neuroblastoma SH-SY5Y cell proliferate and promotes apoptosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 01
Page:
13-17
Research Field:
Publishing date:

Info

Title:
Silencing of Y-box binding protein-1 suppresses neuroblastoma SH-SY5Y cell proliferate and promotes apoptosis
Author(s):
Wang Hong; Zhang Kun; Li Shuang; Chi Zuofei; Wang Runan; Hao Liangchun
Hematology Treatment Center，Pediatric Hematology Department，Shengjing Hospital of China Medical University，Liaoning Shenyang 110022，China.
Keywords:
Y-box binding protein-1; shRNA; neuroblastoma; Cyclin; apoptosis
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2018.01.004
Abstract:
Objective：To investigate the influence of short hairpin RNA (shRNA) mediated silencing YB-1 on neuroblastoma SH-SY5Y cell proliferation and apoptosism then discuss regulatory mechanism.Methods：Establishing the small interfering RNA (siRNA) eukaryotic expression vector targeting YB-1 of human neuroblastoma SH-SY5Y cells，transfection was performed using LipofectamineTM2000 liposome.The expression of YB-1 protein was detected with Western blot after interference.The proliferation of SY5Y cells were detected by MTT assay.The apoptosis of cells was analyzed by flow cytometry.Detected the expression of CyclinD1，Bcl-2 and Bax by Western blot.Results：Expression of YB-1 in transfected group （YB-1 shRNA group） was lower than control group （SH-SY5Y group) (P<0.05).There was no significant difference between control group and invalid transfected group （NC group）.MTT assay showed that cell proliferation level of YB-1 shRNA group was significantly lower than SH-SY5Y group.NC group was similar to SH-SY5Y group.The number of apoptotic cells in YB-1 shRNA group (23.21±1.90)% was higher than SH-SY5Y group (5.05±0.83)% (P<0.01).There was no significant difference between NC group (6.24±1.05)% and SH-SY5Y group (P>0.05) detected by flow cytometry analysis.The level of CyclinD1 and Bcl-2 protein in YB-1 shRNA group was lower than SH-SY5Y group.The level of Bax protein in YB-1 shRNA group was more than SH-SY5Y group.NC group was similar to SH-SY5Y group that detected by Western blot.Conclusion：shRNA mediated silencing YB-1 suppressed neuroblastoma SH-SY5Y cell to proliferate and promoted apoptosis by down regulating CyclinD1 and Bcl-2，as well as up regulating Bax.

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Memo

Memo:
辽宁省自然科学基金项目（编号：2015020542）
Last Update: 2017-11-30