|Table of Contents|

Expressions of intestine-specific transcription factor CDX2 and SRY-related high mobility group-box gene 9 (SOX9) in intestinal metaplasia and gastric cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 05
Page:
760-765
Research Field:
Publishing date:

Info

Title:
Expressions of intestine-specific transcription factor CDX2 and SRY-related high mobility group-box gene 9 (SOX9) in intestinal metaplasia and gastric cancer
Author(s):
Liu Guisheng1Zhou Zihan1Guo Xueyan1Li Wensheng2Zong Wei1
1.Department of Gastroenterology;2.Department of Pathology,Shaanxi Provincal People's Hospital,Shaanxi Xi'an 710068,China.
Keywords:
gastric tumorintestinal metaplasiaCDX2SOX9
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2017.05.023
Abstract:
Objective:To study the expressions of CDX2 and SOX9 protein in intestinal metaplasia (IM) and gastric cancer tissue,and to investigate the roles of CDX2 and SOX9 in the development of IM and gastric carcinogenesis and progression.Methods:24 cases of chronic atrophic gastritis (CAG) with IM,17 cases of paracancerous IM and 50 cases of gastric cancer were selected to the study.HE staining was used to classify IM and gastric cancer,and the expressions of CDX2 and SOX9 protein in different gastric lesions were assessed with immunohistochemistry SP method.The relationships between CDX2,SOX9 expressions and gastric cancer clinical-pathologic significance were analyzed in all gastric cancer patients.Results:CDX2 protein expressional positive rates in CAG with IM group,paracancerous IM group and gastric cancer group were 87.50%,76.47% and 62.00% respectively.CAG with IM group was significant lower than in the gastric cancer group (P<0.05),and there were no significant differences between CAG with IM group and paracancerous IM group,the same as that between paracancerous IM group and gastric cancer group.The intestinal-type gastric cancer was significant higher than the diffuse-type gastric cancer (77.78% vs 43.48%,P=0.013).The expression of CDX2 in gastric cancer was significant associated with tumor TNM staging,differentiation,Lauren classification and the depth of tumor infiltration (P<0.05).SOX9 protein expressional positive rates in CAG with IM group,paracancerous IM group and gastric cancer group were 54.17%,70.59% and 80.00% respectively.CAG with IM group was significant lower than in gastric cancer group (P<0.05).There were no significant differences between CAG with IM group and paracancerous IM group,the same as that between paracancerous IM group and gastric cancer group.The intestinal-type gastric cancer was significant higher than the diffuse-type gastric cancer (92.59% vs 65.22%,P=0.040).The expression of SOX9 in gastric cancer was significantly associated with tumor differentiation,TNM staging,Lauren classification and lymph nodes metastasis (P<0.05).The positive expressions of CDX2 were negatively correlated with that of SOX9 in gastric cancer (r=-0.391,χ2=5.778,P=0.016).Conclusion:CDX2 and SOX9 may play important roles in the development,progression of IM and gastric carcinogenesis,and probably can be used as a specific predictive index for IM and intestinal-type gastric cancer.CDX2 and SOX9 may also play important roles in the progression,infiltration,metastasis of gastric cancer,and combined dectection of the two factors may serve as valuable markers to selecte suitable therepy method and evaluate the prognosis of gastric cancer.

References:

[1]Liu GS,Gong J,Cheng P,et al.Expression of intestine-specific transcription factor CDX2 in different subtypes of intestinal metaplasia and gastric carcinoma[J].Cancer,2006,25(2):185-189.[刘贵生,龚均,程鹏,等.肠特异性转录因子CDX2在不同亚型肠化生及胃癌组织中的表达[J].癌症,2006,25(2):185-189.]
[2]Blache P,van de Wetering M,Duluc I,et al.SOX9 is an intestine crypt transcription factor,is regulated by the Wnt pathway,and represses the CDX2 and MUC2 genes[J].J Cell Biol,2004,166(1):37-47.
[3]Sun D,Wu Y,Xin Y.The significance and expression of SOX2 and CDX2 protein in gastric carcinoma and precancerous lesions[J].Morden Oncology,2016,24(10):1585-1591.[孙丹,武洋,辛彦.SOX2和CDX2基因编码蛋白在胃癌及其癌前病变中的表达及意义[J].现代肿瘤医学,2016,24(10):1585-1591.]
[4]Yasui W,Sentani K,Sakamoto N,et al.Molecular pathology of gastric cancer:Research and practice[J].Pathol Res Pract,2011,207(10):608-612.
[5]Busuttil RA,Boussioutas A.Intestinal metaplasia:a premalignant lesion involved in gastric carcinogenesis[J].J Gastroenterol Hepatol,2009,24(2):193-201.
[6]Xie Y,Li L,Wang X,et al.Overexpression of Cdx2 inhibits progression of gastric cancer in vitro[J].Int J Oncol,2010,36(2):509-516.
[7]Lian C,Wang XT,Xie YB,et al.Effects of CDX2 overexpression on growth and metastesis of transplanted tumor of human gastric cancer in Nude mice[J].Cancer Research on Prevention and Treatment,2012,39(4):400-402.[廉超,王晓通,谢玉波,等.CDX2 过表达对裸鼠人胃癌移植瘤生长和转移的影响[J].肿瘤防治研究,2012,39(4):400-402.]
[8]Zhang JF,Zhang JG,Kuai XL,et al.Reactivation of the homeotic tumor suppressor gene CDX2 by 5-aza-2'-deoxycytidine-induced demethylation inhibits cell proliferation and induces caspase-independent apoptosis in gastric cancer cells[J].Exp Ther Med,2013,5(3):735-741.
[9]Qian XF,Zhang JF,Yu ZH,et al.Nuclear transcription factor CDX2 inhibits migration and invasion of gastric cancer cells by reversing epithelial-mesenchymal transition[J].Chin J Gastroenterol,2014,19(10):583-587.[钱雪芬,张健锋,俞智华,等.核转录因子CDX2抑制胃癌细胞迁移、侵袭与逆转上皮-间质转化有关[J].胃肠病学,2014,19(10):583-587.]
[10]Masood MA,Loya A,Yusuf MA.CDX2 as a prognostic marker in gastric cancer[J].Acta Gastroenterol Belg,2016,79(2):197-200.
[11]Santos JC,Carrasco-Garcia E,García-Puga M,et al.SOX9 elevation acts with canonical WNT signaling to drive gastric cancer progression[J].Cancer Res,2016,28(08):1120.
[12]Fang YH,Xu FY,Huang Q,et al.Expression of SOX9 in colorectal cancer and its significance[J].J Practical Oncol,2008,23(5):425-428.[方义湖,徐芳英,黄琼,等.SOX9 在结直肠癌中的表达及其意义[J].实用肿瘤杂志,2008,23(5):425-428.]
[13]Zhou CJ,Guo JQ,Zhu KX,et al.Elevated expression of SOX9 is related with the progression of gastric carcinoma[J].Diagnostic Cytopathology,2011,39(2):105-109.
[14]Lv XT,Huang RJ.SOX9 expression in gastric cancer tissue and its relation with prognosis[J].Chin J General Surg,2014,23(6):842-844.[吕新厅,黄仁俊.胃癌组织中SOX9 的表达及其与预后关系[J].中国普通外科杂志,2014,23(6):842-844.]
[15]Serizawa T,Hirata Y,Hayakawa Y,et al.Gastric metaplasia induced by helicobacter pylori is associated with enhanced SOX9 expression via interleukin-1 signaling[J].Infect Immun,2015,84(2):562-572.
[16]Hu XR,Yang WJ,Wu XY,et al.Expression of SOX9 and CDX2 in colorectal cancer[J].Chin J Surg of Integrated Traditional and Western Med,2011,17(6):564-567.[胡向荣,杨文君,吴翔燕,等.SOX9和CDX2在结直肠癌中的表达[J].中国中西医结合外科杂志,2011,17(6):564-567.]
[17]Yasui W,Oue N,Sentani K,et al.Transcriptome dissection of gastric cancer:identification of novel diagnostic and therapeutic targets from pathology specimens[J].Pathology International,2009,59(3):121-136.

Memo

Memo:
陕西省自然科学基金重点项目(编号:2012JZ4003)
Last Update: 2017-01-26