|Table of Contents|

Screening of miRNAs in TGF-β1-induced epithelial-to-mesenchymal transition in breast cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 05
Page:
676-680
Research Field:
Publishing date:

Info

Title:
Screening of miRNAs in TGF-β1-induced epithelial-to-mesenchymal transition in breast cancer cells
Author(s):
Wang Xiaohua1; 2; Song Shuang2; Han Ruijie2; Zuo Kuiyang2; Li Guiqin3; Chu Yanhui2; Liu Haifeng1; 2
1.Laboratory of Pathogenic Microbiology and Immunology，Mudanjiang Medical University，Heilongjiang Mudanjiang 157011，China； 2.Heilongjiang Province Key Laboratory for Anti-fibrosis Biotherapy，Mudanjiang Medical University，Heilongjiang Mudanjiang 157011，China；3.Department of Nephrology of Hongqi Hospital，Mudanjiang Medical University，Heilongjiang Mudanjiang 157011，China.
Keywords:
transforming growth factor-β1(TGF-β1); microRNAs(miRNAs); epithelial-mesenchymal transition (EMT); breast cancer
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2017.05.002
Abstract:
Objective：To obtain the optimal concentration of TGF-β1 and screen for miRNAs that potentially involved in TGF-β1-induced epithelial-mesenchymal transition (EMT) in MCF-7 breast cells.Methods：Epithelial-mesenchymal transition (EMT) in MCF-7 breast cells was induced by different concentrations of TGF-β1 for 48h.The morphology of MCF-7 cells under TGF-β1 treatment was observed by optical microscope.The EMT-related genes in MCF-7 cells were further determined by Real-time PCR and Western blot，and then several EMT-related miRNAs were selected to be verified by Real-time PCR.Results：The morphology in MCF-7 cells treated with TGF-β1 for 48h was shift from cobble to spindle-like shape.The results of Real-time PCR showed that the expression of Vimentin，par6，Col4A1，α-SMA and FN were upregulated，while the E-cadherin was downregulated after treated with TGF-β1 at 2，5 and 10ng/ml.Western blot results showed that the E-cadherin expression was downregulated，while the Vimentin and α-SMA expression were upregulated.Meanwhile，these results showed that the TGF-β1 at 10ng/ml exhibited a more significant effect than at 2 and 5ng/ml.In addition，the miR-16，miR-196a，miR-196b and miR-21 expressions were significantly upregulated after 10ng/ml TGF-β1 treatment for 48h.Conclusion：The EMT model of MCF-7 cell is successfully induced by TGF-β1 at 10ng/ml for 48h.The miR-16，miR-196a，miR-196b and miR-21 expressions are upregulated in TGF-β1-induced EMT cell model，which demonstrated that these miRNAs are closely associated with TGF-β1-induced EMT.

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Memo

Memo:
国家自然科学基金（编号：81500471）；黑龙江省卫生厅项目（编号：2014-212）；牡丹江医学院科学技术研究项目（编号：2011-13）
Last Update: 2017-01-26