|Table of Contents|

Long noncoding RNA HOTAIR inhibits the chemosensitivity to adriamycin of bladder urinary tract epithelial carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 05
Page:
673-676
Research Field:
Publishing date:

Info

Title:
Long noncoding RNA HOTAIR inhibits the chemosensitivity to adriamycin of bladder urinary tract epithelial carcinoma
Author(s):
Zhang Hui1Shang Chao2Guo Yan3Song Yongsheng1
1.Department of Urinary Surgery of the Affiliated Shengjing Hospital,China Medical University,Liaoning Shenyang 110122,China; 2.Department of Neurobiology of Basic Medical College,China Medical University,Liaoning Shenyang 110122,China;3.Liaoning Province Key Laboratory of Oral Medicine,School of Stomatology,China Medical University,Liaoning Shenyang 110122,China.
Keywords:
bladder urinary tract epithelial carcinomalong noncoding RNAHOTAIRadriamycin
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2017.05.001
Abstract:
Objective:To research the expression of lncRNA HOTAIR in bladder urinary tract epithelial carcinoma and its regulation for chemosensitivity to adriamycin.Methods:The expression of HOTAIR in bladder urinary tract epithelial carcinoma tissues and homologous peri-cancerous tissues were detected by fluorescence quantitive PCR.The expression vector and silence vector for HOTAIR were transfected into bladder urinary tract epithelial carcinoma J82 cells,and the transfected effects were detected by fluorescence quantitive PCR.The influence of HOTAIR expression changes on cell proliferation and chemosensitivity to adriamycinin in J82 cells were detected by MTT assay.Results:The results of fluorescence quantitive PCR discovered that,in contrast with homologous peri-cancerous tissues,the HOTAIR expressions increased markedly in bladder urinary tract epithelial carcinoma tissues.The expression and silence vector for HOTAIR could up-regulate and silence the HOTAIR expression respectively in J82 cells.In J82 cells,HOTAIR over-expression promoted cell proliferation,amd inhibited chemosensitivity to adriamycin.Silence of HOTAIR showed opposite regulative effects.Conclusion:Long noncoding RNA HOTAIR was high-expressed in bladder urinary tract epithelial carcinoma,it acted as an oncogene to advance cell proliferation,inhibite chemosensitivity to adriamycin in bladder urinary tract epithelial carcinoma cells.

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Memo

Memo:
国家自然科学基金(编号:81301834,81172408,30901480)
Last Update: 2017-01-26