|Table of Contents|

Effect of Gambogic acid on proliferation and apoptosis in 786-O cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 04
Page:
531-535
Research Field:
Publishing date:

Info

Title:
Effect of Gambogic acid on proliferation and apoptosis in 786-O cells
Author(s):
Wang Jing; Liu Tao; Guo Chunhua; Zhang Yaya; Liu Lei; Wan Youfeng
Department of Oncology/Hematology,the No.174 Hospital of the Chinese People's Liberation Army,the Chenggong Hospital Affiliated to Xiamen University,Fujian Xiamen 361001,China.
Keywords:
Gambogic acid; 786-O cells; apoptosis
PACS:
R737.12
DOI:
10.3969/j.issn.1672-4992.2017.04.007
Abstract:
Objective:To observe the effect of Gambogic acid on proliferation and apoptosis of human renal cell carcinoma 786-O cells,and its possible mechanism.Methods:MTT assay and flow cytometry technology were used to detect the function of Gambogic acid on proliferation and apoptosis of 786-O cells respectively.After treating with Gambogic acid,expression level of Bcl-2,Bax,p65,and IκB in 786-O cells were determined by Western blotting assay,and the NF-κB activity was monitored by fluorescence microscopy.Results:Gambogic acid showed an significant activity of proliferation inhibition on 786-O cells by dose-dependence after 48h,and the IC50 value was （3.4 ± 0.3）μmol/L.Accompany with proliferation inhibition,Gambogic acid was also able to induce the apoptosis of 786-O cells effectively by dose-dependence,and the apoptosis rate reached at （68.1±3.6）% when the concentration was 4.0μmol/L.Western blotting result found that Gambogic acid can down-regulate the expression levels of Bcl-2 protein and up-regulate the expression levels of Bax protein in 786-O cells respectively.Meanwhile,it showed an resistant to IκB degradation which induced by TNF-α-IκB.Fluorescence microscopy assay further confirmed that Gambogic acid was able to prevent NF-κB translocating into the nucleus.Conclusion:Gambogic acid could induce the apoptosis of 786-O cells,and the activity inhibition on NF-κB signaling pathways may contribute to the apoptosis mechanism.

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Last Update: 2016-12-29