|Table of Contents|

Effect of Gambogic acid on proliferation and apoptosis in 786-O cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2017 04
Research Field:
Publishing date:


Effect of Gambogic acid on proliferation and apoptosis in 786-O cells
Wang JingLiu TaoGuo ChunhuaZhang YayaLiu LeiWan Youfeng
Department of Oncology/Hematology,the No.174 Hospital of the Chinese People's Liberation Army,the Chenggong Hospital Affiliated to Xiamen University,Fujian Xiamen 361001,China.
Gambogic acid786-O cellsapoptosis
Objective:To observe the effect of Gambogic acid on proliferation and apoptosis of human renal cell carcinoma 786-O cells,and its possible mechanism.Methods:MTT assay and flow cytometry technology were used to detect the function of Gambogic acid on proliferation and apoptosis of 786-O cells respectively.After treating with Gambogic acid,expression level of Bcl-2,Bax,p65,and IκB in 786-O cells were determined by Western blotting assay,and the NF-κB activity was monitored by fluorescence microscopy.Results:Gambogic acid showed an significant activity of proliferation inhibition on 786-O cells by dose-dependence after 48h,and the IC50 value was (3.4 ± 0.3)μmol/L.Accompany with proliferation inhibition,Gambogic acid was also able to induce the apoptosis of 786-O cells effectively by dose-dependence,and the apoptosis rate reached at (68.1±3.6)% when the concentration was 4.0μmol/L.Western blotting result found that Gambogic acid can down-regulate the expression levels of Bcl-2 protein and up-regulate the expression levels of Bax protein in 786-O cells respectively.Meanwhile,it showed an resistant to IκB degradation which induced by TNF-α-IκB.Fluorescence microscopy assay further confirmed that Gambogic acid was able to prevent NF-κB translocating into the nucleus.Conclusion:Gambogic acid could induce the apoptosis of 786-O cells,and the activity inhibition on NF-κB signaling pathways may contribute to the apoptosis mechanism.


[1] Chen Y,Zheng Y,Xu LF.Everolimus treated anti-RCC current research progress[J].J Liaoning University,2011,38(1):60-66.[陈烨,郑颖,徐利锋.依维莫司抗肾细胞癌研究进展[J].辽宁大学学报,2011,38(1):60-66.]
[2] Song SQ,Wu ZJ,Wang C,et al.Renal carcinoma related factor is up-regulated in high Fuhrman grade renal cell carcinoma and affects prognosis in patients[J].Academic J Second Military Medical University,2014,35(5):566-568.[宋尚卿,吴震杰,王承,等.RCRF在高级别肾癌组织中表达上调并影响肾癌患者预后[J].第二军医大学学报,2014,35(5):566-568.]
[3] Yan X,Fang ZJ,Zheng J,et al.Diagnosis,treatment and prognosis of 254 cases of renal cell carcinoma[J].Tumor,2004,24(3):248-250.[燕翔,方祖军,郑捷,等.254例肾细胞癌的诊治及预后[J].肿瘤,2004,24(3):248-250.]
[4] Win AZ.Renal cell carcinoma metastasis to the gallbladder detected by FDG-PET/CT[J].J Clin Med Res,2014,6(6):482-486.
[5] Humphries LS,Baluch DA,Nystrom LM,et al.Interfascicular renal cell carcinoma metastasis to the ulnar nerve:A case report[J].Hand (N Y),2016,11(2):NP1-4.
[6] Cao Q,Wang J,Zhang M,et al.Genetic variants in RKIP are associated with clear cell renal cell carcinoma risk in a Chinese population[J].PLoS One,2014,9(10):e109285.
[7] Zhang H,Lei Y,Yuan P,et al.ROS-mediated autophagy induced by dysregulation of lipid metabolism plays a protective role in colorectal cancer cells treated with gambogic acid[J].PLoS One,2014,9(5):e96418.
[8] Liu S,Zhao C,Yang C,et al.Gambogic acid suppresses pressure overload cardiac hypertrophy in rats[J].Am J Cardiovasc Dis,2013,3(4):227-238.
[9] Lin YW,Lee LM,Lee WJ,et al.Melatonin inhibits MMP-9 transactivation and renal cell carcinoma metastasis by suppressing Akt-MAPKs pathway and NF-kappa B DNA-binding activity[J].J Pineal Res,2016,60(3):277-290.
[10] Choi KE,Hwang CJ,Gu SM,et al.Cancer cell growth inhibitory effect of bee venom via increase of death receptor 3 expression and inactivation of NF-kappa B in NSCLC cells[J].Toxins(Basel),2014,6(8):2210-2228.
[11] Kankaya D,Kiremitci S,Tulunay O,et al.Gelsolin,NF-kappa B,and p53 expression in clear cell renal cell carcinoma:Impact on outcome[J].Pathol Res Pract,2015,211(7):505-512.
[12] Samarghandian S,Nezhad MA,Mohammadi G.Role of caspases,Bax and Bcl-2 in chrysin-induced apoptosis in the A549 human lung adenocarcinoma epithelial cells[J].Anticancer Agents Med Chem,2014,14(6):901-909.
[13] Wang WL,Tao YP,Han XL,et al.Role of polymorphisms in BCL-2 and BAX genes in modulating the risk of developing non-Hodgkin lymphoma[J].Leuk Lymphoma,2013,55(7):1602-1608.
[14] Mahindhoratep S,Bouda HA,El Shafey N,et al.NF-κB related transgene expression in mouse tibial cranial muscle after pDNA injection followed or not by electrotransfer[J].Biochim Biophys Acta,2014,1840(11):3257-3263.
[15] Cabal-Hierro L,Rodriguez M,Artime N,et al.TRAF-mediated modulation of NF-κB and JNK activation by TNFR2[J].Cell Signal,2014,26(12):2658-2666.
[16] Meffert MK,Chang JM,Wiltgen BJ,et al.NF-kappa B functions in synaptic signaling and behavior[J].Nat Neurosci,2003,6(10):1072-1078.
[17] Dai C,Li B,Zhou Y,et al.Curcumin attenuates quinocetone induced apoptosis and inflammation via the opposite modulation of Nrf2/HO-1 and NF-κB pathway in human hepatocyte L02 cells[J].Food Chem Toxicol,2016,95:52-63.
[18] Kim BW,More SV,Yun YS,et al.A novel synthetic compound MCAP suppresses LPS-induced murine microglial activation in vitro via inhibiting NF-κB and p38 MAPK pathways[J].Acta Pharmacol Sin,2016,37(3):334-343.
[19] Rolhion N,Furniss RC,Grabe G,et al.Inhibition of nuclear transport of NF-κB p65 by the salmonella type III secretion system effector SpvD[J].PLoS Pathog,2016,12(5):e1005653.
[20] Gallagher SJ,Mijatov B,Gunatilake D,et al.Control of NF-κB activity in human melanoma by bromodomain and extra-terminal protein inhibitor I-BET151[J].Pigment Cell Melanoma Res,2014,27(6):1126-1137.
[21] Yang XC,Wang X,Luo L,et al.RNA interference suppression of A100A4 reduces the growth and metastatic phenotype of human renal cancer cells via NF-κB-dependent MMP-2 and Bcl-2 pathway[J].Eur Rev Med Pharmacol Sci,2013,17(12):1669-1680.
[22] Wang Q,Liu S,Tang Y,et al.MPT64 protein from Mycobacterium tuberculosis inhibits apoptosis of macrophages through NF-κB-miRNA21-Bcl-2 pathway[J].PLoS One,2014,9(7):e100949.


Last Update: 2016-12-29