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Construction and identification of the prokaryotic expression recombinant plasmid pUC57-CTP-PTEN

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2017 04
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Construction and identification of the prokaryotic expression recombinant plasmid pUC57-CTP-PTEN
Yu Bei1Huang Yuan2Hu Haifeng1Wang Zili1Yang Jin1
1.Department of Urology;2.Department of Clinical Laboratory,Affiliated Hospital of Chengdu University,Sichuan Chengdu 610081,China.
plasmid pUC57CTPPTENconstructionidentification
Objective:To construct prokaryotic expression recombinant plasmid pUC57-CTP-PTEN of CTP gene and PTEN gene,and prepare for the follow-up study of its function.Methods:Gene CTP-PTEN was synthesized and cloned into the prokaryotic expression vector pUC57 to construct the recombinant plasmid pUC57-CTP-PTEN.Then it was transformed into E.coli DH5α.The recombinant plasmid was identified by restriction enzyme digestion and DNA sequencing.The expression product was identified by Western blotting assay.Results:Gene CTP-PTEN was successfully synthesized.The recombinant plasmid pUC57-CTP-PTEN was identified that it was successfully transformed into E.coli DH5α and expressed by restriction enzyme digestion,DNA sequencing and Western blotting.Conclusion:The recombinant plasmid pUC57-CTP-PTEN was successfully constructed and expressed in E.coli DH5α.


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Last Update: 2016-12-29