|Table of Contents|

Construction and identification of the prokaryotic expression recombinant plasmid pUC57-CTP-PTEN

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 04
Page:
528-530
Research Field:
Publishing date:

Info

Title:
Construction and identification of the prokaryotic expression recombinant plasmid pUC57-CTP-PTEN
Author(s):
Yu Bei1; Huang Yuan2; Hu Haifeng1; Wang Zili1; Yang Jin1
1.Department of Urology;2.Department of Clinical Laboratory,Affiliated Hospital of Chengdu University,Sichuan Chengdu 610081,China.
Keywords:
plasmid pUC57; CTP; PTEN; construction; identification
PACS:
R730.1
DOI:
10.3969/j.issn.1672-4992.2017.04.006
Abstract:
Objective:To construct prokaryotic expression recombinant plasmid pUC57-CTP-PTEN of CTP gene and PTEN gene,and prepare for the follow-up study of its function.Methods:Gene CTP-PTEN was synthesized and cloned into the prokaryotic expression vector pUC57 to construct the recombinant plasmid pUC57-CTP-PTEN.Then it was transformed into E.coli DH5α.The recombinant plasmid was identified by restriction enzyme digestion and DNA sequencing.The expression product was identified by Western blotting assay.Results:Gene CTP-PTEN was successfully synthesized.The recombinant plasmid pUC57-CTP-PTEN was identified that it was successfully transformed into E.coli DH5α and expressed by restriction enzyme digestion,DNA sequencing and Western blotting.Conclusion:The recombinant plasmid pUC57-CTP-PTEN was successfully constructed and expressed in E.coli DH5α.

References:

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Memo

Memo:
成都市医学科研课题（编号：2014001）；成都学院校青年基金项目（编号：2014XJZ08）；成都大学附属医院院内课题（编号：2014016）
Last Update: 2016-12-29