|Table of Contents|

Clinical significance of abnormal tissue RAGE and serum sRAGE expression in colorectal cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 01
Page:
91-95
Research Field:
Publishing date:

Info

Title:
Clinical significance of abnormal tissue RAGE and serum sRAGE expression in colorectal cancer
Author(s):
-
1.Tumor Department;2.Pathology Department;3.Cell Biology Lab.,Jinzhou Central Hospital,Liaoning Jinzhou 121001,China.
Keywords:
colorectal cancerRAGEsRAGE
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2017.01.025
Abstract:
Objective:To investigate the correlation and the clinical significance of the expression of the receptor of advanced glycation end product (RAGE) and the soluble RAGE (sRAGE) in colorectal cancer tissue.Methods:To study the expression of RAGE on colorectal carcinoma cells,pathological paraffin blocks of 49 colorectal cancer patients without diabetes mellitus were enrolled in this study,and 10 adjacent tissues (cancer tissue edge ≥5cm) as control group.The expression of RAGE was analyzed by immunehistochemical staining.To detect the concentration of serum sRAGE,peripheral blood of 49 colorectal cancer patients were collected at about 24 hours before operation and 7th day after surgery.And the peripheral bloods of 30 healthy donors were collected as control group.The serum of blood samples were separated,and the concentrations of serum sRAGE were analyzed by ELISA.Results:The expression of colorectal cancer tissue RAGE (IHS:1.24±0.48) was significantly higher than that of control group (IHS:0.50±0.25) (P<0.05),and the stage Ⅲ was significantly higher than stageⅠ (1.01±0.35) (P<0.05) and Ⅱ (1.11±0.42) (P<0.05).The RAGE expression of poorly differentiated colorectal cancer tissue (IHS:1.58±0.49) was significantly higher than that of moderately differentiated tissues (IHS:1.08±0.45) and well-differentiated tissues (IHS:1.02±0.27) (P<0.05).The concentration of serum sRAGE after radical colorectal cancer resection (265.43±76.85) was below preoperative level (344.77±68.06) (P<0.05),and both of them were higher than that of healthy donors (149.97±30.12) (P<0.05),but was no associated with TNM and pathological differentiation.The preoperative serum sRAGE concentration of higher RAGE expression patients (415.02±85.08)ng/L is higher than that of moderate level RAGE expression patients (334.26±44.56)ng/L and lower level RAGE expression patients (335.95±78.48)ng/L (P<0.05).Conclusion:RAGE expression was negatively related to colorectal cancer differentiation,and positively correlated trends with TNM.Serum sRAGE concentration was positively correlated with colorectal cancer tissue RAGE expression,but no associated with TNM and pathological differentiation.RAGE examination for the diagnosis of colorectal cancer after surgery has clinical significance,but the serum sRAGE examination before surgery for colorectal cancer diagnosis can only show a colorectal cancer risk.

References:

[1]Schmidt AM,Vianna M,Gerlach M,et al.Isolation and characterization of two binding proteins for advanced glycosylation endproducts from bovine lung which are present on the endothelial cell surface[J].Biol chem,1992,267:14987-14997.
[2]Armando Rojas,Ileana González,Erik Morales,et al.Diabetes and cancer:Looking at the multiligand/RAGE axis[J].World J Diabetes,2011,2(7):108-113.
[3]Rojas A,Figueroa H,Morales E,et al.Fueling inflammation at tumor microenvironment:the role of multiligand/RAGE axis[J].Carcinogenesis,2010,31:334-341.
[4]Varun Parkash Singh,Anjana Bali,Nirmal Singh,et al.Advanced glycation end products and diabetic complications[J].Korean J Physiol Pharmacol,2014,18:1-14.
[5]Hoffmann S,Friedrichs U,Eichler W,et al.Advanced glycation end products induce choroidal endothelial cell proliferation,matrix metalloproteinase-2 and VEGF upregulation in vitro[J].Graefes Arch Clin Exp Ophthalmol,2002,240:996-1002.
[6]Zhang WY,Ke J,Mao QS,et al.Differential expression of receptor for advanced glycation end products in the tissues of colon adenocarcinoma and normal colon[J].Jiangsu Med J,2012,38(3):315-317.[章文毅,柯靖,毛勤生,等.RAGE在结肠癌和正常结肠组织的表达差异[J].江苏医药,2012,38(3):315-317.]
[7]DiNorcia J,Moroziewicz DN,Ippagunta N,et al.RAGE signaling significantly impacts tumorigenesis and hepatic tumor growth in murine models ofcolorectal carcinoma[J].J Gastrointest Surg,2010,14(11):1680-1690.
[8]Liang H,Zhong Y,Zhou S,et al.Knockdown of RAGE expression inhibits colorectal cancer cell invasion and suppresses angiogenesis in vitro and in vivo[J].Cancer Lett,2011,313(1):91-98.
[9]Dahlmann M,Okhrimenko A,Marcinkowski P,et al.RAGE mediates S100A4-induced cell motility via MAPK/ERK and hypoxia signaling and is a prognostic biomarker for human colorectal cancer metastasis[J].Oncotarget,2014,5(10):3220-3233.
[10]Jing R,Cui M,Wang J,et al.Receptor for advanced glycation end products(RAGE)soluble form(sRAGE):a new biomarker for lung cancer[J].Neoplasma,2010,57(1):55-61.
[11]Tesarová P,Kalousová M,Jáchymová M,et al.Receptor for advanced glycation end products(RAGE)-soluble form(sRAGE)and gene polymorphisms in patients with breast cancer[J].Cancer Invest,2007,25(8):720-725.
[12]Wittwer C,Boeck S,Heinemann V,et al.Soluble receptor of advanced glycation end products(sRAGE)indicates response to chemotherapy in pancreatic cancer patients[J].Int J Clin Pharmacol Ther,2013,51(1):67-69.
[13]Tam HL,Shiu SW,Wong Y,et al.Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes[J].Atherosclerosis,2010,209:173-177.
[14]Xie WX,Wu XY.Clinical significance of HMGB1/RAGE expression in esophageal squamous carcinoma[J].China practical medicine,2010,6(4):6-8.[谢文熙,吴心愿.HMGB1/RAGE蛋白在食管鳞癌中的表达及临床意义[J].中国实用医药,2010,6(4):6-8.]
[15]Huang JJ,Wang BQ,Su BZ,et al.Correlation of receptor of advanced glycation end products and angiogenesis in nasopharyngeal carcinoma[J].China J Mod Drug,2010,4(3):2-4.[黄家军,王斌全,苏炳泽,等.鼻咽癌组织RAGE表达与微血管发生的相关性研究[J].中国现代药物应用,2010,4(3):2-4.]
[16]Takada M,Hirata K,Ajiki T,et al.Expression of receptor for advanced glycation end products (RAGE) and MMP-9 in human pancreatic cancer cells[J].Hepatogastroenterol,2004,51(58):928.
[17]Tsung,Klune JR,Zhang X,et al.HMGBl release induced by liver ischemia involves Toll-like receptor 4 dependent reactive oxygen species production and calcium-mediated signaling[J].Exp Med,2007,204(12):2913-2923.
[18]Armando Rojas,Hector Figueroa,Erik Morales.Fueling inflammation at tumor microenvironment:the role of multiligand/rage axis[J].Carcinogenesis,2010,31(3):334-341.
[19]Wang J,Wang H,Shi J,et al.Effects of bone marrow MSCs transfected with sRAGE on the intervention of HMGB1 induced immuno-inflammatory reaction[J].Int J Clin Exp Pathol,2015,8(10):1228-1240.
[20]Yonekura H,Yamamoto Y,Sakurai S,et al.Novel splice variants of the receptor for advanced glycation end-products expressed in human vascular endothelial cells and pericytes,and their putative roles in diabetes-induced vascular injury[J].Bio Chem J,2003,370(Pt3):1097-1109.

Memo

Memo:
辽宁省自然科学基金项目(编号:201202079)
Last Update: 2016-12-01