|Table of Contents|

Study of the mechanism of miRNA-200b induced an increase in blood-tumor barrier permeability

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 01
Page:
1-5
Research Field:
Publishing date:

Info

Title:
Study of the mechanism of miRNA-200b induced an increase in blood-tumor barrier permeability
Author(s):
Ma Teng; Xue Yixue
Department of Neurobiology，College of Basic Medicine，China Medical University，Liaoning Shenyang 110122，China.
Keywords:
miR-200b; blood-brain barrier; blood-tumor barrier; ZO-1; actin rearrangement
PACS:
R739.4
DOI:
10.3969/j.issn.1672-4992.2017.01.001
Abstract:
Objective：To investigate whether the mechanism of microRNA(miRNA）miR-200b nediated an increase in blood-tumor barrier(BTB).Methods：HRP flux and TEER assays revealed blood-brain barrier (BBB）and BTB permeability.Endogenous expression of miR-200b was detected by Real-time PCR in rat cerebral microvascular endothelial cell RCMEC (ECs）of BBB and BTB.miR-200b agomir and miR-200b antagomir were transfected into GECs (ECs with U87 glioma cells co-culturing)，respectively.The protein expression level of ZO-1 was detected by Western blot.The stress fiber formation and distribution of filamentous actin (F-actin）and ZO-1 were assessed by immunofluorescence microscopy.Results：Compared with BBB，the value of TEER significantly decreased in BTB，and HRP flow significantly increased in BTB，which resulted to an increase in BTB permeability.The endogenous expression of miR-200b in ECs significantly decreased in BTB compared to BBB.miR-200b agomir and miR-200b antagomir were successfully transfected into GECs.Overexpression of miR-200b inhibited endothelial leakage and restored normal transendothelial electric resistance values.Simultaneously，overexpression of miR-200b inhibited a decrease in the protein expression level of ZO-1.In addition，overexpression of miR-200b inhibited the relocation of ZO-1 from cellular borders into the cytoplasm as well as the production of stress fiber formation in GECs of the BTB.miR-200b silencing produced opposite results as that obtained from that of the miR-200b overexpression group.Conclusion：miR-200b mediated an increase in BTB permeability.

References:

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Memo

Memo:
国家自然科学基金项目(编号：81101918，81673028)
Last Update: 2016-12-01