|Table of Contents|

HPV16 infection and p16 protein expression in oropharyngeal squamous cell carcinoma and their clinical significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 13
Page:
1813-1818
Research Field:
Publishing date:

Info

Title:
HPV16 infection and p16 protein expression in oropharyngeal squamous cell carcinoma and their clinical significance
Author(s):
Cui Xiangning1; 2; Zhang Xin1; 2; Xu Jing1; 2; Huang Donghai1; 2; Tang Yaoyun1; 2; Tian Yongquan1; 2; Fan Songqing3; Liu Junwen4
1.Department of Otolaryngology Head and Neck Surgery,Xiangya Hospital of Central South University,Hunan Changsha 410008,China;2.Otolaryngology Major Disease Research Key Laboratory of Hunan Province,Hunan Changsha 410008,China;3.Department of Pathology,Xiangya Second Hospital of Central South University,Hunan Changsha 410011,China;4.Xiangya School of Medicine,Central South University,Hunan Changsha 410013,China.
Keywords:
oropharyngeal squamous cell carcinoma; HPV16; p16; prognosis
PACS:
R739.63
DOI:
10.3969/j.issn.1672-4992.2015.13.09
Abstract:
Objective：To investigate the infection rate of human papillomavirus16 (HPV16) and p16 protein expression in oropharyngeal squamous cell carcinoma (OPSCC) and their clinical significance.Methods：HPV16 DNA was measured by in situ hybridization in 60 specimens of OPSCC.p16 protein was evaluated by immunohistochemistry in 95 specimens.We assessed mutual correlation of the HPV16 and p16,and summarized their clinical significance in oropharyngeal cancer.Results：The incidence rate of HPV infection was 48.3%（29/60）.p16 was expressed in 52.6%（50/95） of the tumor tissues.The p16 protein expression was highly correlated with the presence of HPV16 in 60 specimens （P<0.001).We can use the p16 expression instead of HPV16 infection to predict prognosis alternative in our specimens as the correct prediction rate was 90%（54/60).This was consistent with domestic and international coverage.Compared p16 positive group to the negative group，there was a lower proportion in smokers，drinkers，non-chewing betelers，T3+T4，lymph node metastasis and III-IV clinical stage patients，though the difference was not statistically (P>0.05).The mean follow-up time was 38 months and the follow-up rate was 65.3%（62/95).Both OS and DFS were increased in p16 positive group （70.4% and 63.0%) compared with the p16 negative group (40.0% and 25.7%,P=0.008 and 0.004，respectively) in 62 patients.When multivariable analysis was performed in DFS,patients with p16 protein negative tumors had a higher risk of death than patients with p16 protein positive tumors (HR 0.423，95%CI 0.201-0.891，P=0.024).p16 was closely related to the prognosis of oropharyngeal cancer.Conclusion：The p16 protein expression was highly correlated with the presence of HPV16,and might be a surrogate marker for HPV16 to predict the prognosis.There was a trend that p16 protein positive oropharyngeal cancer patients might be non-smokers，non-drinkers，chewing betelers and low degree of tumor differentiation.Detecting the p16 protein expression has important prognostic significance for OPSCC patients.

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Memo

Memo:
国家自然科学基金(编号：81272974，81201740，81372906，81270371)
Last Update: 2015-05-29