|Table of Contents|

Effect of radiolabeled GMBP1 peptide on cytotoxicity and induction of apoptosis of multiple drugs resistance cells in gastric cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2012 06
Page:
1106-1109
Research Field:
Publishing date:

Info

Title:
Effect of radiolabeled GMBP1 peptide on cytotoxicity and induction of apoptosis of multiple drugs resistance cells in gastric cancer
Author(s):
ZHANG Jing1; LIANG Shuhui1; KANG Jianqin1; LIN Tao2; WANG Biaoluo1; DING Jie1
1.State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Disease, Fourth Military Medical University, Shaanxi Xi'an 710032, China; 2.Department of Digestive Diseases，Chinese PLA 451 Hospital, Shaanxi Xi'an 710054, China.
Keywords:
131I-GMBP1;  apoptosis;  MTT;  peptide;  gastric cancer;  multiple drugs resistance
PACS:
R730.55;R735.2
DOI:
10.3969/j.issn.1672-4992.2012.06.04
Abstract:
Objective：To explore the effect of 131I-GMBP1 on cytotoxicity and induction of apoptosis of multiple drugs resistance (MDR) cells in gastric cancer. Methods: The Iodogen tube method was used to label GMBP1 with 131I. The cytotoxicity of 131I-GMBP1 on gastric cancer MDR cell SGC-7901/ACR was determined by MTT assay. Apoptosis of gastric cancer MDR cell SGC7901/ADR induced by 131I-GMBP1 was investigated by flow cytometry and Hoechst Staining Kit.Results:The radiolabeling efficiency and specific activity of 131I-GMBP1 was 93%-98% and 906.5GBq/mmol, respectively.131I-GMBP1 had remarkable cytotoxic effects on SGC-7901/ACR and could significantly inhibit the growth of SGC-7901/ACR. The IC50 of SGC-7901/ACR was 0.83μg/ml after 96 h exposure to 131I-GMBP1. After 60 h exposure to different concentration of 131I-GMBP1,131I and GMBP1, SGC-7901/ADR treated with 131I-GMBP1 showed pronounced cell apoptosis in a concentration-dependent manner. The highest cell apoptosis rate was 34.1%. The effect of 131I-GMBP1 on the cytotoxicity and induction of apoptosis of gastric cancer MDR cells was dependent on the concentration and time of 131I-GMBP1 exposure. Conclusion:131I-GMBP1 has strong effects on cytotoxicity and induction of apoptosis of gastric cancer MDR cells. These results provide an experimental basis for possible clinical application of 131I-GMBP1 alone or in combination with conventional antineoplastic agents in the treatment of MDR tumors.

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Memo

Memo:
国家自然科学基金资助项目（编号：No. 30900674, No. 30973428, No. 30900704）
Last Update: 2012-06-11