|Table of Contents|

Preliminary study on the effect of intratumoral gram-negative bacteria on lung cancer cell death

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 02
Page:
194-198
Research Field:
Publishing date:

Info

Title:
Preliminary study on the effect of intratumoral gram-negative bacteria on lung cancer cell death
Author(s):
ZHANG Huijie1; ZHAI Meng'en2; JIANG Jianli3; JING Lin3
1.School of Basic Medicine,Air Force Medical University,Shannxi Xi'an 710032,China;2.Department of Cardiovascular Surgery,the First Affiliated Hospital,Air Force Medical University,Shannxi Xi'an 710032,China;3.Department of Cell Biology,National Translational Science Center for Molecular Medicine,Fourth Military Medical University,Shannxi Xi'an 710032,China.
Keywords:
intratumoral microbiota; lung cancer; cell death; RIPK3
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2025.02.005
Abstract:
Objective:To investigate the effect of gram-negative bacteria residing in lung cancer tissue on lung cancer cell death.Methods:Lung cancer cells were treated with different concentrations of lipopolysaccharide (LPS) (0 μg/mL,0.01 μg/mL,0.1 μg/mL,1 μg/mL) in vitro,and the expression of RIPK3 was detected by real-time fluorescent quantitative PCR (RT-qPCR) or Western blot.The microRNA that may regulate RIPK3 was predicted using TargetScan database,and the targeting relationship between the predicted microRNA and RIPK3 was verified by dual luciferase reporter gene assay and Western blot.Lung cancer cells were treated with 0.01 μg/mL LPS and the expression level of miR-637 was detected by RT-qPCR.Lung cancer cells were treated with LPS and/or cisplatin (DDP),and the expression of PARP and cleaved-PARP (cl-PARP) was detected by Western blot.Results:Compared with the control group,the mRNA and protein levels of RIPK3 were significantly up-regulated in LPS (0.01 μg/mL) group (P＜0.05 and P＜0.001).The results of dual luciferase reporter gene assay showed that compared with miR-18a-3p,miR-18b-3p,miR-223-3p,miR-342-3p or miR-874-3p group,the luciferase activity of miR-637 group was significantly decreased compared with that of NC group (P＜0.01).The results of Western blot showed that compared with NC group,the expression of RIPK3 was significantly down-regulated in miR-637 mimics group (P＜0.05),and the expression of RIPK3 was significantly increased in miR-637 inhibitor group when comparing with inhibitor NC group (P＜0.01).Compared with the control group,the expression of miR-637 was significantly down-regulated in cells treated with LPS (0.01 μg/mL) (P＜0.05).PARP protein cleavage was detected in both DDP group and LPS+DDP group,but less cleaved PARP protein was detected in LPS+DDP group.Conclusion:A low dosage of LPS can up-regulate the expression of cell death-related protein RIPK3 in lung cancer cells via decreasing the expression of miR-637,but will inhibit cisplatin-induced apoptosis in cells.

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