|Table of Contents|

Melatonin increases the sensitivity of esophageal cancer cells to paclitaxel by inhibiting the expression of ESCCAL-1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 02
Page:
186-193
Research Field:
Publishing date:

Info

Title:
Melatonin increases the sensitivity of esophageal cancer cells to paclitaxel by inhibiting the expression of ESCCAL-1
Author(s):
ZHANG Jing; ZHANG Yueli; GUAN Hongya; LIU Jia
Zhengzhou Central Hospital Affiliated to Zhengzhou University,Henan Zhengzhou 450007,China.
Keywords:
melatonin; esophageal cancer; paclitaxel; ESCCAL-1
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2025.02.004
Abstract:
Objective:To investigate the effect of melatonin on enhancing sensitivity of esophageal cancer cells to paclitaxel and its mechanism.Methods:Different concentrations of melatonin and paclitaxel were used to treat esophageal cancer cells separately or in combination.The changes of cell proliferation were analyzed by CCK-8 assay and clonogenesis assay.The expression of lncRNA ESCCAL-1 was detected by RT-qPCR.Western blot analysis was used to detect p-AKT,c-MYC,Bcl-2 and ULK1 protein levels.Results:Melatonin inhibited the activity of esophageal cancer cells in a dose-dependent manner (P＜0.05).Melatonin increased the inhibitory effect of paclitaxel on EC-9706 and EC-109 cells (P＜0.05),and decreased its IC50 value (P＜0.01).The expression of ESCCAL-1 was inhibited by 0.5~2 mmol/L of melatonin (P＜0.01),and overexpression of ESCCAL-1 reversed the effect of melatonin on paclitaxel.Melatonin inhibited the activation of AKT pathway,down-regulated the levels of protein c-MYC and Bcl-2,and up-regulated the level of protein ULK1.However,overexpression of ESCCAL-1 reversed the effects of melatonin on the expression of AKT pathway and related proteins.Conclusion:Melatonin inhibits the AKT pathway and cells proliferation by decreasing the expression of ESCCAL-1,and thereby increases the sensitivity of esophageal cancer cells to paclitaxel.

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河南省重点研发与推广专项（科技攻关）（编号：222102310107，232102311058）；河南省医学科技攻关计划省部共建项目（编号：SBGJ202103101）；河南省高校重点科研项目（编号：23A310025，23A310022）
Last Update: 1900-01-01