|Table of Contents|

Effect of miR-93-5p on radiosensitivity of triple-negative breast cancer and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 01
Page:
14-19
Research Field:
Publishing date:

Info

Title:
Effect of miR-93-5p on radiosensitivity of triple-negative breast cancer and its mechanism
Author(s):
GAO Hui1; PAN Chi2; NI Qingtao2; XU Hong2
1.Southeast University Hospital,Jiangsu Nanjing 210009,China;2.The Affiliated Taizhou People's Hospital of Nanjing Medical University,Taizhou School of Clinical Medicine,Nanjing Medical University,Jiangsu Taizhou 225300,China.
Keywords:
triple-negative breast cancer; miR-93-5p; radiosensitivity; GSDME
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2025.01.003
Abstract:
Objective:To explore the effect of miR-93-5p on the radiosensitivity of triple-negative breast cancer(TNBC) and its related mechanism.Methods:Double luciferase experiment showed that GSDME（Gasdermin E） was the target gene of miR-93-5p.Stable overexpression cells of miR-93-5p were constructed,and mice were treated with radiotherapy after subcutaneous tumor formation.The expression of GSDME in each group was detected by real-time fluorescence quantitative PCR(qPCR) and Western Blot(WB).To detect the expression of miR-93-5p and GSDME in cancer tissues and adjacent tissues of TNBC tumor patients,compare their differential expression changes in cancer tissues and adjacent tissues,and analyze their correlation with TNBC clinical pathology.Results:miR-93-5p directly targets GSDME 3'-UTR,and the expression of GSDME was low in the tissues of mice overexpressing miR-93-5p.Radiotherapy promoted the expression of GSDME and blocked the inhibition of miR-93-5p on GSDME.miR-93-5p was mainly located in cytoplasm.GSDME was decreased in tumor tissue of TNBC patients compared with that in adjacent tissues.Conclusion:Radiotherapy promotes GSDME expression and blocks the inhibition of GSDME by miR-93-5p,which has potential as a radiobiological marker.

References:

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Memo

Memo:
江苏省双创博士项目（编号：202003205，202003206）；南京医科大学泰州临床医学院科研项目-国自然（青年基金）培育专项（编号：TZKY20220105，TZKY20220109）
Last Update: 1900-01-01