|Table of Contents|

Study on the mechanism of zinc oxide nanoparticles inhibiting the cell proliferation,angiogenesis and lung metastasis of osteosarcoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 01
Page:
7-13
Research Field:
Publishing date:

Info

Title:
Study on the mechanism of zinc oxide nanoparticles inhibiting the cell proliferation,angiogenesis and lung metastasis of osteosarcoma
Author(s):
HE Guanping
Department of Orthopedics,Beijing Chao-Yang Hospital,Capital Medical University,Beijing 100020,China.
Keywords:
zinc oxide nanoparticles; osteosarcoma; proliferation; tumor angiogenesis; lung metastasis
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2025.01.002
Abstract:
Objective:To investigate the effect of zinc oxide nanoparticles on proliferation,angiogenesis and lung metastasis of osteosarcoma in vitro and in vivo.Methods:The SaoS-2 and 143B osteosarcoma cell lines were divided into the experimental group and control group.The experimental group was treated with zinc oxide nanoparticles of different concentrations(10 μmol/L,20 μmol/L).After 24 hours,the cell proliferation activity was detected by CCK-8 and clone formation assay，the ability of angiogenesis was detected by tube forming experiment,the expression of VEGF-a protein was detected by Western blot.The growth,lung metastasis and survival of tibial osteosarcoma in vivo were evaluated in nude mouse model,HE staining and immunohistochemistry were used to detect the expression of VEGF-a and Ki-67 protein in tumor tissues.Results:CCK-8 experiment showed that the proliferation inhibition rate of SaoS-2 and 143B osteosarcoma cells in the zinc oxide nanoparticles treatment group was significantly lower than that in the control group from the third day,and showed a time and concentration dependent manner.Clone formation experiment also showed that on the 10th day,the number of cell communities of SaoS-2 and 143B osteosarcoma cells treated with zinc oxide nanoparticles was significantly less than that of the control group.The results of tube forming experiment showed that compared with the control group,zinc oxide nanoparticles could significantly inhibit the angiogenesis of human umbilical vein endothelial cells.Western blot results showed that the expression of VEGF-a protein in osteosarcoma cells treated with zinc oxide nanoparticles was significantly downregulated.The results of in vivo model of tibial osteosarcoma in nude mice showed that the zinc oxide nanoparticles treatment group significantly inhibited the growth of tibial osteosarcoma in situ and lung metastasis,the expression of VEGF-a and Ki-67 proteins in tumor tissue but prolonged the survival time of nude mice.Conclusion:Zinc oxide nanoparticles can inhibit the proliferation,angiogenesis and improve the prognosis,and its mechanism may be related to the downregulation of VEGF-a protein expression.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.82203893）；国家自然科学基金（编号：82203893）
Last Update: 1900-01-01