|Table of Contents|

Expression of GPX8 in breast cancer and its clinical significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 05
Page:
848-856
Research Field:
Publishing date:

Info

Title:
Expression of GPX8 in breast cancer and its clinical significance
Author(s):
LI He1; ZHAO Yanchun2; HAN Jianjun3; ZHUO Jian1; DAI Luxian4; SHENG Ankang4; YAO Hanyu4; LIU Weiguang4
1.School of Clinical Medicine,Hebei University of Engineering,Hebei Handan 056002,China;2.Department of Outpatient;3.Department of Breast Surgery,Affiliated Hospital of Hebei University of Engineering,Hebei Handan 056002,China;4.Department of Mammary Gland,Yangzhou Maternal and Child Health Hospital,Yangzhou University,Jiangsu Yangzhou 225002,China.
Keywords:
breast cancer; GPX8; prognosis; TILs; TNBC
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.05.012
Abstract:
Objective:We utilized bioinformatics analysis,immunohistochemistry,and clinical cohort analysis to predict,analyze,and assess the differential expression,diagnostic potential,and prognostic significance of GPX8 in the context of breast cancer.Methods:We downloaded GPX8 gene expression,RNAseq,DNA methylation,survival,phenotype,dry score,and clinical information data of patients with cancer from the UCSC Xena database and conducted a Kaplan-Meier survival prognosis analysis,and explored methylation levels.The correlation between GPX8 and tumor-infiltrating lymphocytes in breast cancer was evaluated using the tumor immune estimation resource(TIMER),and the potential functional pathways affected by GPX8 were analyzed using gene set enrichment analysis(GSEA).A total of 125 patients who were diagnosed with breast cancer underwent immunohistochemical and HE staining,followed by clinical follow-up analysis of GPX8.GPX8 expression levels and prognostic relationships in different subgroups were analyzed using the Chi-square test and Cox regression.Results:Gene expression profiling interaction analysis(GEPIA) and TIMER analysis revealed that GPX8 mRNA expression in patients with breast cancer was higher in para-cancer tissues,and the prognosis was significantly different.GPX8 protein expression was highest in triple-negative breast cancer.In the TIMER database,the mRNA expression of GPX8 was found to be negatively correlated with the infiltration of CD8+T,B cells,NK,and Treg cells.Furthermore,within the clinical cohort,an observation has been made indicating a positive correlation between GPX8 mRNA expression and factors such as TNM stage,T stage,and molecular subtypes.Additionally,in patients with triple-negative breast cancer,the expression levels of GPX8 were found to be negatively correlated with tumor-infiltrating lymphocytes(TILs).Patients with high GPX8 expression had significantly reduced overall survival and an increased risk of death.Conclusion：GPX8 is expressed differently in breast cancer tissues and can be used as an effective prognostic biomarker for clinical diagnosis.

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Memo

Memo:
河北省自然科学基金（编号：H2021402015）
Last Update: 2024-01-30