|Table of Contents|

Effect of butorphanol on malignant biological behavior of bladder cancer cells by regulating PI3K/AKT/mTOR signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 05
Page:
805-810
Research Field:
Publishing date:

Info

Title:
Effect of butorphanol on malignant biological behavior of bladder cancer cells by regulating PI3K/AKT/mTOR signaling pathway
Author(s):
WU Xiudong1; WEI Tangqiang2; YU Xiaoqian1
1.Anesthesia Operation Center;2.Department of Urology,Affiliated Hospital of Chengdu University,Sichuan Chengdu 610081,China.
Keywords:
bladder cancer; butorphanol; PI3K/AKT/mTOR; proliferation; migration; invasion
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2024.05.004
Abstract:
Objective:To investigate the effect of butorphanol on the malignant biological behavior of bladder cancer cells by regulating the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR) signaling pathway.Methods:Bladder cancer cell T24 was cultured in vitro.Cells were grouped into control group,low-dose butorphanol group(1 ng/mL),medium-dose butorphanol group(10 ng/mL),high-dose butorphanol group(100 ng/mL),activator group(100 ng/mL butorphanol+PI3K agonist Recilisib 10 μmol/L),and inhibitor group(100 ng/mL butorphanol+PI3K inhibitor LY294002 50 μmol/L).CCK-8 experiment was applied to detect cell proliferation.Flow cytometry was applied to detect cell apoptosis.Transwell was applied to detect cell migration and invasion.Western blot was applied to detect the expression of PI3K/AKT/mTOR signaling pathway related proteins in cells and a xenograft tumor model was constructed,the tumor mass was weighed,and the tumor volume was calculated.Results:Compared with the control group,the OD450 value of T24 cells,the numbers of migrating and invading cells,and the expression of p-PI3K,p-AKT,and p-mTOR proteins in the low-dose,medium dose,and high-dose butorphanol groups were obviously reduced,the apoptosis rate of cells was obviously increased,in a dose-dependent manner(P＜0.05).Activators weakened the inhibitory effect of butorphanol on T24 cell proliferation,migration,and invasion,promoting cell apoptosis,while inhibitors strengthened the inhibitory effect of butorphanol on T24 cell proliferation,migration,and invasion,and the promoting effect on cell apoptosis.Compared with nude mice in the control group,the tumor volume and mass in the low dose,medium dose,and high dose butorphanol groups were obviously reduced,in a dose-dependent manner(P＜0.05).Compared with nude mice in high-dose butorphanol group,the tumor volume and mass in the nude mouse activator group were obviously increased(P＜0.05),while the tumor volume and mass in the nude mouse inhibitor group were obviously reduced(P＜0.05).Conclusion:Butorphanol inhibits the proliferation,migration and invasion of bladder cancer cells by inhibiting PI3K/AKT/mTOR signaling pathway.

References:

[1]COMPERAT E,AMIN MB,CATHOMAS R,et al.Current best practice for bladder cancer:a narrative review of diagnostics and treatments［J］.Lancet,2022,400(10364):1712-1721.
[2] RIGHETTO M,MODONUTTI D,CELSO FG,et al.Metabolic syndrome and bladder cancer［J］.Panminerva Med,2022,64(3):316-323.
[3] ZHANG Y,XIE S,ZHANG G,et al.Analgesic effect of butorphanol tartrate combined with hydromorphone on patients with cesarean section:A prospective cohort study［J］.Medicine(Baltimore),2022,101(48):e31901.
[4] 刘辉,王雅琳,李福龙,等.布托啡诺抑制TLR4/MyD88/NF-κB信号通路对卵巢癌细胞增殖、凋亡、迁移和侵袭的影响［J］.中国优生与遗传杂志,2022,30(7):1107-1111. LIU H,WANG YL,LI FL,et al.Influences of butorphanol on the proliferation,apoptosis,migration and invasion of ovarian cancer cells by inhibiting TLR4/MyD88/NF-κB signaling pathway［J］.Chinese Journal of Birth Health & Heredity,2022,30(7):1107-1111.
[5] CUI P,XIN D,LI F,et al.Butorphanol suppresses the proliferation and migration of osteosarcoma by promoting the expression of piRNA hsa_piR_006613［J］.Front Oncol,2022,12(1):1-10.
[6] YU L,WEI J,LIU P.Attacking the PI3K/Akt/mTOR signaling pathway for targeted therapeutic treatment in human cancer［J］.Semin Cancer Biol,2022,85(1):69-94.
[7] CIRONE M.Cancer cells dysregulate PI3K/AKT/mTOR pathway activation to ensure their survival and proliferation:mimicking them is a smart strategy of gammaherpesviruses［J］.Crit Rev Biochem Mol Biol,2021,56(5):500-509.
[8] CHEN K,ZHU P,CHEN W,et al.Melatonin inhibits proliferation,migration,and invasion by inducing ROS-mediated apoptosis via suppression of the PI3K/Akt/mTOR signaling pathway in gallbladder cancer cells［J］.Aging(Albany NY),2021,13(18):22502-22515.
[9] 王迎春,高继奎,刘璐.酒石酸布托啡诺对宫颈癌细胞增殖、凋亡的影响及机制研究［J］.中国实验诊断学,2022,26(12):1834-1838. WANG YC,GAO JK,LIU L.The effects and mechanism of butorphanol tartrate on the proliferation and apoptosis of cervical cancer cells［J］.Chinese Journal of Laboratory Diagnosis,2022,26(12):1834-1838.
[10] LIU J,XU H,WANG N,et al.miR-15b,a diagnostic biomarker and therapeutic target,inhibits oesophageal cancer progression by regulating the PI3K/AKT signalling pathway［J］.Exp Ther Med,2020,20(6):1-10.
[11] 徐余超,陈晓伟,孙海翔,等.LY294002抑制PI3K/AKT/mTOR信号通路对胃癌细胞MGC-803的影响［J］.环境与职业医学,2017,34(5):403-409. XU YC,CHEN XW,SUN HX,et al.Effects of inhibiting PI3K/AKT/mTOR signaling pathway by LY294002 on gastric cancer MGC-803 cells［J］.Journal of Environmental and Occupational Medicine,2017,34(5):403-409.
[12] 刘锦源,司林杰.酒石酸布托啡诺预处理对小鼠心肌缺血再灌注损伤的保护作用及其机制［J］.东南大学学报(医学版),2017,36(1):74-77. LIU JY,SI LJ.Effects of butorphanol pretreatment on myocardial ischemia-reperfusion injury and it's mechanisms in mice［J］.Journal of Southeast University(Medical Science Edition),2017,36(1):74-77.
[13] GONG Z,LAO D,WU Y,et al.Inhibiting PI3K/Akt-signaling pathway improves neurobehavior changes in anti-NMDAR encephalitis mice by ameliorating blood-brain barrier disruption and neuronal damage ［J］.Cell Mol Neurobiol,2023,10(1):1-15.
[14] LOBO N,AFFERI L,MOSCHINI M,et al.Epidemiology,screening,and prevention of bladder cancer［J］.Eur Urol Oncol,2022,5(6):628-639.
[15] TRAN L,XIAO JF,AGARWAL N,et al.Advances in bladder cancer biology and therapy［J］.Nat Rev Cancer,2021,21(2):104-121.
[16] GUO P,HU Q,WANG J,et al.Butorphanol inhibits angiogenesis and migration of hepatocellular carcinoma and regulates MAPK pathway［J］.Antibiot(Tokyo),2022,75(11):626-634.
[17] 刘彦立.布托啡诺调控PBX3基因抑制乳腺癌细胞系MCF7增殖、迁移和侵袭［J］.基础医学与临床,2020,40(2):203-208. LIU YL.Butorphanol inhibits the proliferation,migration and invasion of cell line MCF7 through regulating PBX3 gene［J］.Basic and Clinical Medicine,2020,40(2):203-208.
[18] WANG B,LI Y,SHEN Y,et al.Butorphanol inhibits the malignant biological behaviors of ovarian cancer cells via down-rgulating the expression of TMEFF1［J］.Onco Targets Ther,2020,13(1):10973-10981.
[19] WANG J,HU K,CAI X,et al.Targeting PI3K/AKT signaling for treatment of idiopathic pulmonary fibrosis［J］.Acta Pharm Sin B,2022,12(1):18-32.
[20] CHENG Q,CHEN M,LIU M,et al.Semaphorin 5A suppresses ferroptosis through activation of PI3K-AKT-mTOR signaling in rheumatoid arthritis［J］.Cell Death Dis,2022,13(7):1-10.
[21] 徐利本，吴朝阳，王远东.PI3K/Akt信号传导通路在肿瘤发生发展及治疗中的作用 ［J］.现代肿瘤医学，2021,29(01):177－180. XU LB,WU CY,WANG YD.The role of PI3K/Akt signal pathway in the development and progression of tumor［J］.Modern Oncology,2021,29(01):177－180.
[22] XIAO Y,LIU Y,GAO Z,et al.Fisetin inhibits the proliferation,migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling［J］.Aging(Albany NY),2021,13(22):24753-24767.

Memo

Memo:
四川省科技计划项目(编号：2023JDRC0095)
Last Update: 2024-01-30