|Table of Contents|

Silencing circRNA hsa_circ_0061137 inhibits cervical cancer cell growth and metastasis through the miR-217/ARL6IP1 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 03
Page:
435-442
Research Field:
Publishing date:

Info

Title:
Silencing circRNA hsa_circ_0061137 inhibits cervical cancer cell growth and metastasis through the miR-217/ARL6IP1 axis
Author(s):
ZHANG Xiaoli; DONG Li; DU Chenxu; CUI Lingling; ZHANG Shi; XIE Linsen
Department of Clinical Laboratory,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Henan Zhengzhou 450001,China.
Keywords:
circular RNA hsa_circ_0061137; miR-217/ARL6IP1 signal axis; cervical cancer; growth; metastasis
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.03.008
Abstract:
Objective:To investigate the effect of circRNA hsa_circ_0061137 on the growth and metastasis of cervical cancer and its mechanism.Methods:CC tissue samples（93 cases),adjacent tissue samples（93 cases),normal cervical epithelial cell lines（End1/E6E7) and CC cell lines（HeLa,SiHa,C-33A,CaSki) were taken.qRT-PCR was used to detect the expression of hsa_circ_0061137,miR-217,ARL6IP1 in tissues and cells.Dual luciferase reporter test was used to verify the targeted regulation of hsa_circ_0061137,ARL6IP1 and miR-217.After knocking down hsa_circ_0061137 in CC cell lines（HeLa,SiHa) and co-transfecting with si-hsa_circ_0061137 and miR-217 inhibitor in HeLa cells.CCK8 method was used to detect cell proliferation.Flow cytometry was used to detect cell apoptosis.Transwell method was used to detect cell migration and invasion.Western blot was used to detect EMT-related proteins（E-cadherin,Vimentin).qRT-PCR method was used to detect the expression of hsa_circ_0061137,miR-217,ARL6IP1 in each group of cells.Tumor-bearing test in nude mice was used to detect the effect of knocking down hsa_circ_0061137 on tumor growth.Results:The expression levels of hsa_circ_0061137 and ARL6IP1 in CC tissues and cell lines were significantly higher than those in normal cervical tissues and normal cervical epithelial cells（P＜0.05).The expression level of miR-217 in CC tissues and cell lines was significantly lower than that of normal cervical tissues and normal cervical epithelial cells（P＜0.05).There was a targeted negative regulatory relationship between hsa_circ_0061137,ARL6IP1 and miR-217,respectively.Knockdown of hsa_circ_0061137 could inhibit CC cell proliferation,migration,invasion and EMT characteristics,and promote CC cell apoptosis（P＜0.05).miR-217 inhibitor could partially reverse the anti-CC growth and metastasis effects of hsa_circ_0061137 knockdown（P＜0.05).The nude mouse tumor-bearing test confirmed that hsa_circ_0061137 knockdown could significantly reduce tumor growth and proliferation,and inhibit the expression of ARL6IP1（P＜0.05).Conclusion:Silencing hsa_circ_0061137 can play an anti-CC proliferation and metastasis role,and its action may be related to the target miR-217/ARL6IP1 axis.

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河南省高等学校重点科研项目（编号：21A310029）
Last Update: 2022-12-30