|Table of Contents|

Rapid identification of human hepatocellular carcinoma cells and normal liver cells by surface-enhanced Raman spectroscopy based on gold nanoparticles

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 03
Page:
423-427
Research Field:
Publishing date:

Info

Title:
Rapid identification of human hepatocellular carcinoma cells and normal liver cells by surface-enhanced Raman spectroscopy based on gold nanoparticles
Author(s):
ZHOU Qing1; 2; LU Feng1; 2
1.Naval Military Medical University,Shanghai 200433,China;2.Pharmaceutical Analysis Laboratory,School of Pharmacy,Naval Military Medical University,Shanghai 200433,China.
Keywords:
hepatocellular carcinoma cells; surface-enhanced Raman spectroscopy; gold nanoparticles; tumor cells; principal component analysis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.03.006
Abstract:
Objective:To perform rapid and accurate identification of normal hepatocytes and different types of hepatocellular carcinoma cells using gold nanoparticle-based surface-enhanced Raman spectroscopy（SERS).Methods:Liver cancer cells were cultured in vitro and gold nanoparticles were added to the culture medium for 30 min.Accordingly,human hepatocellular carcinoma cells were determined using SERS.Results:The results revealed that gold nanoparticle-based SERS could rapidly detect and identify human hepatocellular carcinoma cells,including human hepatocellular carcinoma cells HepG2（RSD 1.20%),hepatic cholangiocarcinoma cells HCCC-9810（RSD 11.14%),human hepatoblastoma cells HuH-6（RSD 9.50%),human hepatocellular carcinoma cells SK-Hep（RSD 4.60%),and human normal hepatocytes HL7702（RSD 4.50%).These cell types were then compared with other tumour cells.Finally,peak assignments were performed on the SERS spectra.Conclusion:This investigation conclude that gold nanoparticle-based SERS is functionally capable of accurate and rapid identification of human hepatocellular carcinoma cells.

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Memo

Memo:
National Natural Science Foundation of China（No.82273894）;国家自然科学基金面上项目（编号：82273894）；上海市军民融合发展专项（编号：2020-jmrh1-kj1102）
Last Update: 2022-12-30