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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 01

Page:

50-57

Research Field:

Publishing date:

Info

Title:

The effect mechanism of resveratrol against T-cell acute lymphoblastic leukemia by Wnt/¦Â-catenin signaling pathway

Author(s):

SHI Min¹; 2; LI Yiyao¹; KANG Peiying¹; WANG Shaohua¹; 2; WANG Wenjing¹; ZHANG Yuanyuan¹;  LI Yongjun¹; 2

1.Department of Clinical Laboratory,Second Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China;2.Key Laboratory of Laboratory Medicine of Hebei Province£¬Hebei Shijiazhuang 050000£¬China.

Keywords:

resveratrol; T-cell acute lymphoblastic leukemia; Molt-4 cell; Jurkat cell; T-ALL mice models; Wnt/¦Â-catenin signaling pathway

PACS:

R733.71

DOI:

10.3969/j.issn.1672-4992.2023.01.008

Abstract:

Objective:To explore the roles and possible mechanisms of Wnt/¦Â-catenin signaling pathway in resveratrol against T-cell acute lymphoblastic leukemia (T-ALL) in vitro and in vivo by cellular level and animal model.Methods:Human T-ALL cell lines (Molt-4 cells and Jurkat cells) were divided into control group,DMSO group and resveratrol treated group.C57BL/6 mice were randomly divided into three groups,including control group,T-ALL group and T-ALL mice with resveratrol treated group (resveratrol group).CCK-8 assay was performed to detect cell activity in Molt-4 cells and Jurkat cells.Mice injected with ICN1-GFP+ T-ALL cells by tail vein injection were used to establish the T-ALL model.The percentage of ICN1-GFP+ T-ALL cells in peripheral blood of mice was detected by flow cytometry.Real-time quantitative PCR (RT-PCR) was used to detect the expressions of c-Myc and Cyclin D1 in T-ALL cells and mice spleen tissues.The expressions of ¦Â-catenin,TCF-1,LEF-1,c-Myc and Cyclin D1 in T-ALL cell lines and mice spleen were detected by Western Blot.Results:The cell activity of Molt-4 cells and Jurkat cells was significantly suppressed after treating with resveratrol (P£¼0.01),and the inhibition effect was strengthened with the increasing dose of resveratrol (P£¼0.01).After treating with resveratrol,the expression of c-Myc and Cyclin D1 mRNA were significantly reduced (P£¼0.01).The protein expressions of ¦Â-catenin,TCF-1,LEF-1,c-Myc and Cyclin D1 in resveratrol group were reduced significantly (P£¼0.05).In vivo experiments,compared with the control group,the protein expressions of ¦Â-catenin,TCF-1,LEF-1,c-Myc,Cyclin D1,and the mRNA expression of c-Myc and Cyclin D1 were increased significantly in the mice spleen of T-ALL group (P£¼0.01).After resveratrol administration,the percentage of ICN1-GFP+ T-ALL cells in the peripheral blood of mice decreased notably (P£¼0.01) and the protein expression of ¦Â-catenin,TCF-1,LEF-1,c-Myc,Cyclin D1,and the mRNA expression of c-Myc,Cyclin D1 were significantly reduced in resveratrol group (P£¼0.01).Conclusion£ºIn T-ALL cell lines and animal models,resveratrol may play an anti-acute T-lymphoblastic leukemia role by down-regulating the expression of ¦Â-catenin,TCF-1 and LEF-1 in the Wnt/¦Â-catenin signaling pathway and then inhibiting the revels of traget proteins c-Myc and Cyclin D1.

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Last Update: 2022-11-30