［1］PROSSER SL,PELLETIER L. Mitotic spindle assembly in animal cells:a fine balancing act［J］. Nat Rev Mol Cell Biol,2017,18(3):187-201.
［2］YAMADA M,GOSHIMA G. Mitotic spindle assembly in land plants:Molecules and mechanisms［J］. Biology (Basel),2017,6(1) :pii:E6.
［3］XIE S,ZHOU J. Harnessing plant biodiversity for the discovery of novel anticancer drugs targeting microtubules［J］. Front Plant Sci,2017,8:720.
［4］MASOUD K,HERZOG E,CHABOUTE ME,et al. Microtubule nucleation and establishment of the mitotic spindle in vascular plant cells［J］. Plant J,2013,75(2):245-257.
［5］XIE S,OGDEN A,ANEJA R,et al. Microtubule-binding proteins as promising biomarkers of paclitaxel sensitivity in cancer chemotherapy［J］. Med Res Rev,2016,36(2):300-312.
［6］HE X,LIU Z,HE Q,et al. Identification of novel microtubule-binding proteins by taxol-mediated microtubule stabilization and mass spectrometry analysis［J］. Thorac Cancer,2015,6(5):649-654.
［7］WANG Y,SMALLWOOD PM,WILLIAMS J,et al. A mouse model for kinesin family member 11 (Kif11)-associated familial exudative vitreoretinopathy［J］. Hum Mol Genet,2020,29(7):1121-1131.
［8］LU W,GELFAND VI. Moonlighting motors:kinesin,dynein,and cell polarity［J］. Trends Cell Biol,2017,27(7):505-514.
［9］VENUTO S,MONTEONOFRIO L,COZZOLINO F,et al. TRIM8 interacts with KIF11 and KIFC1 and controls bipolar spindle formation and chromosomal stability［J］. Cancer Lett,2020,473:98-106.
［10］HIROKAWA N,TANAKA Y. Kinesin superfamily proteins (KIFs):Various functions and their relevance for important phenomena in life and diseases［J］. Exp Cell Res,2015,334(1):16-25.
［11］WANG W,CAO L,WANG C,et al. Kinesin,30 years later:Recent insights from structural studies［J］. Protein Sci,2015,24(7):1047-1056.
［12］WAITZMAN JS,RICE SE. Mechanism and regulation of kinesin-5,an essential motor for the mitotic spindle［J］. Biol Cell,2014,106(1):1-12.
［13］HATA S,PASTOR PEIDRO A,PANIC M,et al. The balance between KIFC3 and EG5 tetrameric kinesins controls the onset of mitotic spindle assembly［J］. Nat Cell Biol,2019,21(9):1138-1151.
［14］GILLMOR CS,ROEDER AH,SIEBER P,et al. A genetic screen for mutations affecting cell division in the arabidopsis thaliana embryo identifies seven loci required for cytokinesis［J］. PLoS One,2016,11(1):e0146492.
［15］MIKI T,NAITO H,NISHINA M,et al. Endogenous localizome identifies 43 mitotic kinesins in a plant cell［J］. Proc Natl Acad Sci USA,2014,111(11):E1053-1061.
［16］LEE YR,QIU W,LIU B. Kinesin motors in plants:from subcellular dynamics to motility regulation［J］. Curr Opin Plant Biol,2015,28:120-126.
［17］PEI YY,LI GC,RAN J,et al. Kinesin family member 11 enhances the self-renewal ability of breast cancer cells by participating in the Wnt/beta-catenin pathway［J］. J Breast Cancer,2019,22(4):522-532.
［18］LIU M,WANG X,YANG Y,et al. Ectopic expression of the microtubule-dependent motor protein Eg5 promotes pancreatic tumourigenesis［J］. J Pathol,2010,221(2):221-228.
［19］CHEN GY,MICKOLAJCZYK KJ,HANCOCK WO. The Kinesin-5 chemomechanical cycle is dominated by a two-heads-bound state［J］. J Biol Chem,2016,291(39):20283-20294.
［20］SHIMAMOTO Y,FORTH S,KAPOOR TM. Measuring pushing and braking forces generated by ensembles of kinesin-5 crosslinking two microtubules［J］. Dev Cell,2015,34(6):669-681.
［21］MURETTA JM,JUN Y,GROSS SP,et al. The structural kinetics of switch-1 and the neck linker explain the functions of kinesin-1 and Eg5［J］. Proc Natl Acad Sci USA,2015,112(48):E6606-6613.
［22］FERNANDEZ JP,AGUERO TH,VEGA LOPEZ GA,et al. Developmental expression and role of Kinesin Eg5 during Xenopus laevis embryogenesis［J］. Dev Dyn,2014,243(4):527-540.
［23］EIBES S,GALLISA-SUNE N,ROSAS-SALVANS M,et al. Nek9 phosphorylation defines a new role for tpx2 in eg5-dependent centrosome separation before nuclear envelope breakdown［J］. Curr Biol,2018,28(1):121-129 e124.
［24］MENG XQ,DAI YY,JING LD,et al. Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice［J］. J Reprod Dev,2016,62(4):351-358.
［25］HARA-YOKOYAMA M,KURIHARA H,ICHINOSE S,et al. KIF11 as a potential marker of spermatogenesis within mouse seminiferous tubule cross-sections［J］. J Histochem Cytochem,2019,67(11):813-824.
［26］BISHOP JD,HAN Z,SCHUMACHER JM. The Caenorhabditis elegans aurora B kinase AIR-2 phosphorylates and is required for the localization of a BimC kinesin to meiotic and mitotic spindles［J］. Mol Biol Cell,2005,16(2):742-756.
［27］ZHAO C,QIU J,AGARWAL G,et al. Genome-wide discovery of microsatellite markers from diploid progenitor species,arachis duranensis and a. ipaensis,and their application in cultivated peanut (a. hypogaea)［J］. Front Plant Sci,2017,8:1209.
［28］DUAN Y,HUO D,GAO J,et al. Ubiquitin ligase RNF20/40 facilitates spindle assembly and promotes breast carcinogenesis through stabilizing motor protein Eg5［J］. Nat Commun,2016,7:12648.
［29］NALAWANSHA DA,GOMES ID,WAMBUA MK,et al. HDAC inhibitor-induced mitotic arrest is mediated by eg5/kif11 acetylation［J］. Cell Chem Biol,2017,24(4):481-492 e485.
［30］VAN REE JH,NAM HJ,JEGANATHAN KB,et al. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes［J］. Nat Cell Biol,2016,18(7):814-821.
［31］XIE W,YANG Y,GAO S,et al. The tumor suppressor CYLD controls epithelial morphogenesis and homeostasis by regulating mitotic spindle behavior and adherens junction assembly［J］. J Genet Genomics,2017,44(7):343-353.
［32］CORRECTION FOR MURETTA.A posttranslational modification of the mitotic kinesin Eg5 that enhances its mechanochemical coupling and alters its mitotic function［J］. Proc Natl Acad Sci USA,2018,115(11):E2665.
［33］LUO Y,RAN J,XIE S,et al. ASK1 controls spindle orientation and positioning by phosphorylating EB1 and stabilizing astral microtubules［J］. Cell Discov,2016,2:16033.
［34］LIU Y,WANG Y,DU Z,et al. Fbxo30 regulates mammopoiesis by targeting the bipolar mitotic kinesin Eg5［J］. Cell Rep,2016,15(5):1111-1122.
［35］LIU M,XIE S,ZHOU J. Use of animal models for the imaging and quantification of angiogenesis［J］. Exp Anim,2018,67(1):1-6.
［36］XIE W,ZHOU J. Aberrant regulation of autophagy in mammalian diseases［J］. Biol Lett,2018,14(1):20170540.
［37］YANG Y,MU T,LI T,et al. Effects of FSTL1 on the proliferation and motility of breast cancer cells and vascular endothelial cells［J］. Thorac Cancer,2017,8(6):606-612.
［38］DU X,ZHOU J. Application of biosensors to detection of epidemic diseases in animals［J］. Res Vet Sci,2018,118:444-448.
［39］YANG HT,ZOU SS,ZHAI LJ,et al. Pathogen invasion changes the intestinal microbiota composition and induces innate immune responses in the zebrafish intestine［J］. Fish Shellfish Immunol,2017,71:35-42.
［40］CHEN Y,HANCOCK WO. Kinesin-5 is a microtubule polymerase［J］. Nat Commun,2015,6:8160.
［41］LIU M,LI D,SUN L,et al. Modulation of Eg5 activity contributes to mitotic spindle checkpoint activation and Tat-mediated apoptosis in CD4-positive T-lymphocytes［J］. J Pathol,2014,233(2):138-147.
［42］CHEN H,CONNELL M,MEI L,et al. The non-motor adaptor HMMR dampens Eg5-mediated forces to preserve the kinetics and integrity of chromosome segregation［J］. Mol Biol Cell,2018,29(7):786-796.
［43］DENG JL,XU YH,WANG G. Identification of potential crucial genes and key pathways in breast cancer using bioinformatic analysis［J］. Front Genet,2019,10:695.
［44］ZHOU J,CHEN WR,YANG LC,et al. KIF11 functions as an oncogene and is associated with poor outcomes from breast cancer［J］. Cancer Res Treat,2019,51(3):1207-1221.
［45］JIN Q,DAI Y,WANG Y,et al. High kinesin family member 11 expression predicts poor prognosis in patients with clear cell renal cell carcinoma［J］. J Clin Pathol,2019,72(5):354-362.
［46］LI S,XUAN Y,GAO B,et al. Identification of an eight-gene prognostic signature for lung adenocarcinoma［J］. Cancer Manag Res,2018,10:3383-3392.
［47］LIU X,LIU X,LI J,et al. Identification and integrated analysis of key biomarkers for diagnosis and prognosis of non-small cell lung cancer［J］. Med Sci Monit,2019,25:9280-9289.
［48］OUE N,SENTANI K,SAKAMOTO N,et al. Molecular carcinogenesis of gastric cancer:Lauren classification,mucin phenotype expression,and cancer stem cells［J］. Int J Clin Oncol,2019,24(7):771-778.
［49］IMAI T,OUE N,SENTANI K,et al. KIF11 is required for spheroid formation by oesophageal and colorectal cancer cells［J］. Anticancer Res,2017,37(1):47-55.
［50］CHEN J,LI S,ZHOU S,et al. Kinesin superfamily protein expression and its association with progression and prognosis in hepatocellular carcinoma［J］. J Cancer Res Ther,2017,13(4):651-659.
［51］YI Y,LIU Y,WU W,et al. Reconstruction and analysis of circRNA miRNA mRNA network in the pathology of cervical cancer［J］. Oncol Rep,2019,41(4):2209-2225.
［52］SHI B,BAO J,LIU Y,et al. Death receptor 6 promotes ovarian cancer cell migration through KIF11［J］. FEBS Open Bio,2018,8(9):1497-1507.
［53］PERUZYNSKA M,PIOTROWSKA K,TKACZ M,et al. Comparative evaluation of new dihydropyrimidine and dihydropyridine derivatives perturbing mitotic spindle formation［J］. Future Med Chem,2018,10(20):2395-2410.
［54］YE XS,FAN L,VAN HORN RD,et al. A novel Eg5 inhibitor (LY2523355) causes mitotic arrest and apoptosis in cancer cells and shows potent antitumor activity in xenograft tumor models［J］. Mol Cancer Ther,2015,14(11):2463-2472.
［55］PAN S,ZHAN Y,CHEN X,et al. Identification of biomarkers for controlling cancer stem cell characteristics in bladder cancer by network analysis of transcriptome data stemness indices［J］. Front Oncol,2019,9:613.
［56］DING S,XING N,LU J,et al. Overexpression of Eg5 predicts unfavorable prognosis in non-muscle invasive bladder urothelial carcinoma［J］. Int J Urol,2011,18(6):432-438.
［57］LIU C,ZHOU N,LI J,et al. Eg5 overexpression is predictive of poor prognosis in hepatocellular carcinoma patients［J］. Dis Markers,2017,2017:2176460.
［58］LIU L,LIU X,MARE M,et al. Overexpression of Eg5 correlates with high grade astrocytic neoplasm［J］. J Neurooncol,2016,126(1):77-80.
［59］JUNGWIRTH G,YU T,MOUSTAFA M,et al. Identification of KIF11 as a novel target in meningioma［J］. Cancers (Basel),2019,11(4):E545.
［60］MYERS SM,COLLINS I. Recent findings and future directions for interpolar mitotic kinesin inhibitors in cancer therapy［J］. Future Med Chem,2016,8(4):463-489.
［61］CARBAJALES C,PRADO MA,GUTIERREZ-DE-TERAN H,et al. Structure-based design of new KSP-Eg5 inhibitors assisted by a targeted multicomponent reaction［J］. Chembiochem,2014,15(10):1471-1480.
［62］LORUSSO PM,GONCALVES PH,CASETTA L,et al. First-in-human phase 1 study of filanesib (ARRY-520),a kinesin spindle protein inhibitor,in patients with advanced solid tumors［J］. Invest New Drugs,2015,33(2):440-449.
［63］MANSOOR UF,ANGELES AR,DAI C,et al. Discovery of novel spiro 1,3,4-thiadiazolines as potent,orally bioavailable and brain penetrant KSP inhibitors［J］. Bioorg Med Chem,2015,23(10):2424-2434.
［64］DHARMAPURI S,PERUZZI D,MARRA E,et al. Intratumor RNA interference of cell cycle genes slows down tumor progression［J］. Gene Ther,2011,18(7):727-733.
［65］LU M,ZHU H,WANG X,et al. The prognostic role of Eg5 expression in laryngeal squamous cell carcinoma［J］. Pathology,2016,48(3):214-218.
［66］SAIJO T,ISHII G,OCHIAI A,et al. Eg5 expression is closely correlated with the response of advanced non-small cell lung cancer to antimitotic agents combined with platinum chemotherapy［J］. Lung Cancer,2006,54(2):217-225.
［67］KATO T,LEE D,HUANG H,et al. Personalized siRNA-nanoparticle systemic therapy using metastatic lymph node specimens obtained with EBUS-TBNA in lung cancer［J］. Mol Cancer Res,2018,16(1):47-57.
［68］POLLEY E,KUNKEL M,EVANS D,et al. Small cell lung cancer screen of oncology drugs,Investigational agents,and gene and microRNA expression［J］. J Natl Cancer Inst,2016,108(10):djw122.
［69］DAIGO K,TAKANO A,THANG PM,et al. Characterization of KIF11 as a novel prognostic biomarker and therapeutic target for oral cancer［J］. Int J Oncol,2018,52(1):155-165.
［70］YAN GR,ZOU FY,DANG BL,et al. Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A,a proteomics study［J］. Proteomics,2012,12(14):2391-2399.
［71］ZHANG W,ZHAI L,LU W,et al. Discovery of novel allosteric eg5 inhibitors through structure-based virtual screening［J］. Chem Biol Drug Des,2016,88(2):178-187.
［72］TRUEBENBACH I,ZHANG W,WANG Y,et al. Co-delivery of pretubulysin and siEG5 to EGFR overexpressing carcinoma cells［J］. Int J Pharm,2019,569:118570.
［73］SUN L,SUN X,XIE S,et al. Significant decrease of ADP release rate underlies the potent activity of dimethylenastron to inhibit mitotic kinesin Eg5 and cancer cell proliferation［J］. Biochem Biophys Res Commun,2014,447(3):465-470.
［74］WISSING MD,DE MORREE ES,DEZENTJE VO,et al. Nuclear Eg5 (kinesin spindle protein) expression predicts docetaxel response and prostate cancer aggressiveness［J］. Oncotarget,2014,5(17):7357-7367.
［75］HAYASHI N,KOLLER E,FAZLI L,et al. Effects of Eg5 knockdown on human prostate cancer xenograft growth and chemosensitivity［J］. Prostate,2008,68(12):1283-1295.
［76］SUN L,LU J,NIU Z,et al. A potent chemotherapeutic strategy with eg5 inhibitor against gemcitabine resistant bladder cancer［J］. PLoS One,2015,10(12):e0144484.
［77］BIGLEY AL,KLEIN SK,DAVIES B,et al. Using automated image analysis algorithms to distinguish normal,aberrant,and degenerate mitotic figures induced by Eg5 inhibition［J］. Toxicol Pathol,2016,44(5):663-672.
［78］LEE DJ,KESSEL E,EDINGER D,et al. Dual antitumoral potency of EG5 siRNA nanoplexes armed with cytotoxic bifunctional glutamyl-methotrexate targeting ligand［J］. Biomaterials,2016,77:98-110.
［79］SUN D,LU J,DING K,et al. The expression of Eg5 predicts a poor outcome for patients with renal cell carcinoma［J］. Med Oncol,2013,30(1):476.
［80］DING S,ZHAO Z,SUN D,et al. Eg5 inhibitor,a novel potent targeted therapy,induces cell apoptosis in renal cell carcinoma［J］. Tumour Biol,2014,35(8):7659-7668.
［81］WANG F,LIN SL. Knockdown of kinesin KIF11 abrogates directed migration in response to epidermal growth factor-mediated chemotaxis［J］. Biochem Biophys Res Commun,2014,452(3):642-648.
［82］REMON J,ABEDALLAA N,TARANCHON-CLERMONT E,et al. CD52,CD22,CD26,EG5 and IGF-1R expression in thymic malignancies［J］. Lung Cancer,2017,108:168-172.